A Golden Gate Modular Cloning Toolbox for Plants
Plant Synthetic Biology requires robust and efficient methods for assembling multigene constructs. Golden Gate cloning provides a precision module-based cloning technique for facile assembly of multiple genes in one construct. We present here a versatile resource for plant biologists comprising a se...
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| Veröffentlicht in: | ACS synthetic biology 2014-11, Vol.3 (11), p.839-843 |
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| container_title | ACS synthetic biology |
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| creator | Engler, Carola Youles, Mark Gruetzner, Ramona Ehnert, Tim-Martin Werner, Stefan Jones, Jonathan D. G Patron, Nicola J Marillonnet, Sylvestre |
| description | Plant Synthetic Biology requires robust and efficient methods for assembling multigene constructs. Golden Gate cloning provides a precision module-based cloning technique for facile assembly of multiple genes in one construct. We present here a versatile resource for plant biologists comprising a set of cloning vectors and 96 standardized parts to enable Golden Gate construction of multigene constructs for plant transformation. Parts include promoters, untranslated sequences, reporters, antigenic tags, localization signals, selectable markers, and terminators. The comparative performance of parts in the model plant Nicotiana benthamiana is discussed. |
| doi_str_mv | 10.1021/sb4001504 |
| format | Article |
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The comparative performance of parts in the model plant Nicotiana benthamiana is discussed.</description><subject>Agrobacterium tumefaciens - genetics</subject><subject>Cloning, Molecular - methods</subject><subject>Genetic Engineering - methods</subject><subject>Genetic Vectors - genetics</subject><subject>Models, Genetic</subject><subject>Nicotiana - genetics</subject><subject>Synthetic Biology - methods</subject><issn>2161-5063</issn><issn>2161-5063</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkE1LAzEQhoMottQe_AOSi6CH1Xxn91iKVqGih3oO2c2stKSbmuyC_nsjreLBucwcHl7eeRA6p-SGEkZvUy0IoZKIIzRmVNFCEsWP_9wjNE1pQ_JIySUvT9GIiYpzysQYkRleBO-gwwvbA34KbvA24rkP3bp7w6sQfB0-cBsifvG269MZOmmtTzA97Al6vb9bzR-K5fPicT5bFpZT2Re8Ik1pWy0sVADSgW5KBVxVlgtVUeWsAqK0qxWUxLJW09zUVkJq4gCc5RN0tc_dxfA-QOrNdp0a8LkEhCEZqpgmWmhGM3q9R5sYUorQml1cb238NJSYb0fm11FmLw6xQ70F90v-GMnA5R6wTTKbMMQuf_lP0BdvRmo9</recordid><startdate>20141121</startdate><enddate>20141121</enddate><creator>Engler, Carola</creator><creator>Youles, Mark</creator><creator>Gruetzner, Ramona</creator><creator>Ehnert, Tim-Martin</creator><creator>Werner, Stefan</creator><creator>Jones, Jonathan D. 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| ispartof | ACS synthetic biology, 2014-11, Vol.3 (11), p.839-843 |
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| subjects | Agrobacterium tumefaciens - genetics Cloning, Molecular - methods Genetic Engineering - methods Genetic Vectors - genetics Models, Genetic Nicotiana - genetics Synthetic Biology - methods |
| title | A Golden Gate Modular Cloning Toolbox for Plants |
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