A Golden Gate Modular Cloning Toolbox for Plants

A Golden Gate Modular Cloning Toolbox for Plants

Plant Synthetic Biology requires robust and efficient methods for assembling multigene constructs. Golden Gate cloning provides a precision module-based cloning technique for facile assembly of multiple genes in one construct. We present here a versatile resource for plant biologists comprising a se...

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Veröffentlicht in:ACS synthetic biology 2014-11, Vol.3 (11), p.839-843
Hauptverfasser: Engler, Carola, Youles, Mark, Gruetzner, Ramona, Ehnert, Tim-Martin, Werner, Stefan, Jones, Jonathan D. G, Patron, Nicola J, Marillonnet, Sylvestre
Format: Artikel
Sprache:eng
Schlagworte:
Agrobacterium tumefaciens - genetics
Cloning, Molecular - methods
Genetic Engineering - methods
Genetic Vectors - genetics
Models, Genetic
Nicotiana - genetics
Synthetic Biology - methods
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Zusammenfassung:Plant Synthetic Biology requires robust and efficient methods for assembling multigene constructs. Golden Gate cloning provides a precision module-based cloning technique for facile assembly of multiple genes in one construct. We present here a versatile resource for plant biologists comprising a set of cloning vectors and 96 standardized parts to enable Golden Gate construction of multigene constructs for plant transformation. Parts include promoters, untranslated sequences, reporters, antigenic tags, localization signals, selectable markers, and terminators. The comparative performance of parts in the model plant Nicotiana benthamiana is discussed.
ISSN:2161-5063
2161-5063
DOI:10.1021/sb4001504