Lipase catalysed isomerisation of 1,2-(2,3)-diglyceride into 1,3-diglyceride. The crucial role of water

Enzyme catalyzed isomerisation of 1,2-dipalmitin into the 1,3-isomer is shown to occur with 1,3 regiospecific lipases. The mechanism of this transformation is elucidated and the crucial role of the water still present in the enzyme preparation is shown. Thus, the first step is the hydrolysis of the...

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Veröffentlicht in:Biotechnology letters 1991-05, Vol.13 (5), p.327-332
Hauptverfasser: HEISLER, A, RABILLER, C, HUBLIN, L
Format: Artikel
Sprache:eng
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Zusammenfassung:Enzyme catalyzed isomerisation of 1,2-dipalmitin into the 1,3-isomer is shown to occur with 1,3 regiospecific lipases. The mechanism of this transformation is elucidated and the crucial role of the water still present in the enzyme preparation is shown. Thus, the first step is the hydrolysis of the dipalmitin followed by the isomerisation of the 2-monopalmitin into the 1-monoester. The last is then esterified into the 1,3-dipalmitin.
ISSN:0141-5492
1573-6776
DOI:10.1007/BF01027677