Molecular cloning and expression in Escherichia coli K‐12 of chromosomal genes determining the O antigen of an E. coli O2: K1 strain

Molecular cloning and expression in Escherichia coli K‐12 of chromosomal genes determining the O antigen of an E. coli O2: K1 strain

: The rfb gene cluster which determines the biosynthesis of the O2 O antigen has been cloned from an Escherichia coli O2: K1 strain isolated from a case of septicaemia in chickens. The region required for expression of the O antigen in E. coli K‐12 was localised to a 10.7 to 14.15‐kb segment which w...

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Veröffentlicht in:FEMS microbiology letters 1991-08, Vol.82 (3), p.345-352
Hauptverfasser: Neal, Brian L., Tsiolis, George C., Heuzenroeder, Michael W., Manning, Paul A., Reeves, Peter R.
Format: Artikel
Sprache:eng
Schlagworte:
Antigen, O
Escherichia coli
Lipopolysaccharide
rfb
Serotype
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Zusammenfassung:: The rfb gene cluster which determines the biosynthesis of the O2 O antigen has been cloned from an Escherichia coli O2: K1 strain isolated from a case of septicaemia in chickens. The region required for expression of the O antigen in E. coli K‐12 was localised to a 10.7 to 14.15‐kb segment which was shown to be chromosomal in origin with a close linkage to the gnd and his genetic loci.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1991.tb04907.x