Validation and Application of a Model of Oxygen Consumption and Diffusion During Photodynamic Therapy In Vitro

Validation and Application of a Model of Oxygen Consumption and Diffusion During Photodynamic Therapy In Vitro

The photophysical parameters for the photosensitizer Pd(II) meso‐Tetra(4‐carboxyphenyl) porphine (PdT790) acquired in a previous study were incorporated into the PDT oxygen diffusion models for cell suspensions and cell monolayers. The time‐dependent phosphorescence signals generated by the diffusio...

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Veröffentlicht in:Photochemistry and photobiology 2014-11, Vol.90 (6), p.1359-1367
Hauptverfasser: Weston, Mark A., Patterson, Michael S.
Format: Artikel
Sprache:eng
Schlagworte:
Diffusion
In Vitro Techniques
Models, Biological
Oxygen
Oxygen Consumption
Photochemotherapy
Spheroids
Time
Validation studies
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Zusammenfassung:The photophysical parameters for the photosensitizer Pd(II) meso‐Tetra(4‐carboxyphenyl) porphine (PdT790) acquired in a previous study were incorporated into the PDT oxygen diffusion models for cell suspensions and cell monolayers. The time‐dependent phosphorescence signals generated by the diffusion models are shown to match signals previously measured by M.A.W. and M.S.P. when reasonable physical and photophysical parameters are used. Simulations were performed to investigate the effects of metabolic and photodynamic oxygen consumption rates on the PDT dose in each of the treatment geometries. It was found that in cell suspensions of
ISSN:0031-8655
1751-1097
DOI:10.1111/php.12320