VMA11, a novel gene that encodes a putative proteolipid, is indispensable for expression of yeast vacuolar membrane H super(+)-ATPase activity

A gene, VMA11, is indispensable for expression of the vacuolar membrane H super(+)-ATPase activity in the yeast Saccharomyces cerevisiae). The VMA11 gene was isolated from a yeast genomic DNA-library by complementation of the vma11 mutation. The nucleotide sequence of the gene predicts a hydrophobic proteolipid of 164 amino acids with a calculated molecular mass of 17,037 daltons. The deduced amino acid sequence shows 56.7% identity, and significant coincidence in amino acid composition with the 16-kDa subunit c (a VMA3 gene product) of the yeast vacuolar membrane H super(+)-ATPase.