Characterization of lysyl residues of NADH-cytochrome b sub(5) reductase implicated in charge-pairing with active-site carboxyl residues of cytochrome b sub(5) by site-directed mutagenesis of an expression vector for the flavoprotein

An expression vector for bovine NADH-cytochrome b sub(5) reductase was constructed from two DNA fragments that were derived from beef liver poly (A super(+)) RNA using the polymerase chain reaction. Site-directed mutagenesis of the 3 lysine residues of the reductase, previously implicated in the for...

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Veröffentlicht in:	The Journal of biological chemistry 1992-01, Vol.267 (4), p.2519-2523
Hauptverfasser:	Strittmatter, P, Kittler, J M, Coghill, JE, Ozols, J
Format:	Artikel
Sprache:	eng
Schlagworte:	catalysis cattle cytochrome b5 reductase liver lysine residues role site-directed mutagenesis
Online-Zugang:	Volltext
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