Formyl peptide stimulates and ATP gamma S potentiates ( super(3)H)cytidine 5'-diphosphate diglyceride accumulation in human neutrophils

Although it is evident that the chemotactic peptide FMLP activates O sub(2) super(-) formation in neutrophils via the phosphoinositidase-mediated second messenger system, it is less clear how endogenous priming agents such as ATP and platelet activating factor potentiate FMLP action. In our study, we have examined the possible effects of the stable ATP analog adenosine 5'-O-(3-thiotriphosphate) (ATP gamma S) on cellular levels of inositol 1,4,5-trisphosphate, (Ca super(2+)) sub(i) and diglyceride (DG), in resting and in FMLP-stimulated neutrophils. It is proposed that the priming of FMLP responses in neutrophils proceeds via a mechanism that selectively enhances DG production through a mechanism that is independent of FMLP-induced breakdown of phosphatidylinositol bisphosphate.