Novobiocin biosynthesis in Streptomyces spheroides: identification of a dimethylallyl diphosphate:4-hydroxyphenylpyruvate dimethylallyl transferase

Abstract A dimethylallyl diphosphate:4-hydroxyphenylpyruvate dimethylallyl transferase from the novobiocin producer Streptomyces spheroides DSM 40292 was identified, partially purified and characterized. The enzyme was soluble, and specific for 4-hydroxyphenylpyruvate and dimethylallyl diphosphate a...

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Veröffentlicht in:	FEMS microbiology letters 1998-04, Vol.161 (1), p.69-74
Hauptverfasser:	Steffensky, Marion, Li, Shu-Ming, Vogler, Bernhard, Heide, Lutz
Format:	Artikel
Sprache:	eng
Schlagworte:	4‐Hydroxyphenylpyruvate Antibacterial agents Antibiotics Antibiotics. Antiinfectious agents. Antiparasitic agents Bacteriology Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest Biosynthesis Biotechnology Fundamental and applied biological sciences. Psychology Medical sciences Metabolism. Enzymes Microbiology Mission oriented research Novobiocin Pharmacology. Drug treatments Physiology and metabolism Prenyltransferase Streptomyces Streptomyces spheroides Substrates
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Zusammenfassung:	Abstract A dimethylallyl diphosphate:4-hydroxyphenylpyruvate dimethylallyl transferase from the novobiocin producer Streptomyces spheroides DSM 40292 was identified, partially purified and characterized. The enzyme was soluble, and specific for 4-hydroxyphenylpyruvate and dimethylallyl diphosphate as substrates. It is most likely involved in the biosynthesis of the prenylated 4-hydroxybenzoate moiety of the aminocoumarin antibiotic novobiocin.
ISSN:	0378-1097 1574-6968
DOI:	10.1111/j.1574-6968.1998.tb12930.x