Note: A micro-perfusion system for use during real-time physiological studies under high pressure

We construct a micro-perfusion system using piston screw pump generators for use during real-time, high-pressure physiological studies. Perfusion is achieved using two generators, with one generator being compressed while the other is retracted, thus maintaining pressurization while producing fluid...

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Veröffentlicht in:Review of scientific instruments 2014-10, Vol.85 (10), p.106106-106106
Hauptverfasser: Maltas, Jeff, Long, Zac, Huff, Alison, Maloney, Ryan, Ryan, Jordan, Urayama, Paul
Format: Artikel
Sprache:eng
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Zusammenfassung:We construct a micro-perfusion system using piston screw pump generators for use during real-time, high-pressure physiological studies. Perfusion is achieved using two generators, with one generator being compressed while the other is retracted, thus maintaining pressurization while producing fluid flow. We demonstrate control over perfusion rates in the 10-μl/s range and the ability to change between fluid reservoirs at up to 50 MPa. We validate the screw-pump approach by monitoring the cyanide-induced response of UV-excited autofluorescence from Saccharomyces cerevisiae under pressurization.
ISSN:0034-6748
1089-7623
DOI:10.1063/1.4899121