Physiology of exolaccase production by Thelephora terrestris

Abstract Thelephora terrestris, an ectomycorrhizal hymenomycete, produces extracellular laccase when grown in minimal liquid medium. The enzyme was characterized as a protein of 66 kDa. The optimal pH varied depending on the substrate utilized, being 5.0 for syringaldazine, 4.8 for guaiacol and 3.4...

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Veröffentlicht in:	FEMS microbiology letters 1998-04, Vol.161 (1), p.151-156
Hauptverfasser:	Kanunfre, Carla C, Zancan, Glaci T
Format:	Artikel
Sprache:	eng
Schlagworte:	Ammonium Ammonium phosphates Biological and medical sciences Ectomycorrhizal fungus Ectomycorrhizas Fundamental and applied biological sciences. Psychology Growth, nutrition, metabolism, transports, enzymes. Molecular biology Guaiacol Laccase Microbiology Mycology Substrates Syringaldazine Thelephora terrestris
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Zusammenfassung:	Abstract Thelephora terrestris, an ectomycorrhizal hymenomycete, produces extracellular laccase when grown in minimal liquid medium. The enzyme was characterized as a protein of 66 kDa. The optimal pH varied depending on the substrate utilized, being 5.0 for syringaldazine, 4.8 for guaiacol and 3.4 for ABTS. The Km was 2.52±0.4 μM for syringaldazine, 16±1.9 μM for ABTS and 120.6±4.9 μM for guaiacol. The secretion of laccase was biomass-dependent and independent of the glucose concentration in the medium. The addition of inducers did not increase laccase production which was dependent upon the ammonium phosphate concentration.
ISSN:	0378-1097 1574-6968
DOI:	10.1111/j.1574-6968.1998.tb12942.x