Evidence for glycosyl-phosphatidylinositol anchoring of intralumenal alkaline phosphatase of the calf intestine
Considerable amounts of intestinal alkaline phosphatase were found intralumenally in all animal species investigated, i.e. calf, pig, goat, rat, mouse, guinea pig, hen and carp. The ratios between the total activity of AP found intralumenally and the total intestinal activity vary considerably. For...
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| Veröffentlicht in: | European journal of biochemistry 1991-01, Vol.199 (2), p.305-312 |
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| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
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| Zusammenfassung: | Considerable amounts of intestinal alkaline phosphatase were found intralumenally in all animal species investigated, i.e. calf, pig, goat, rat, mouse, guinea pig, hen and carp. The ratios between the total activity of AP found intralumenally and the total intestinal activity vary considerably. For the intralumenal alkaline phosphatase of the mucosa and chyme of calf, all criteria were found which are generally used, indicating a glycosyl-phosphatidylinositol (GlcPtdIns) anchor as proved by strong hydrophobicity using Triton X-114 phase partitioning. |
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| ISSN: | 0014-2956 |