PCR method established for the detection of aspergillus fumigatus

Objective To establish a PCR method for the detection of aspergillus fumigatus. Methods Design a pair of special primers during the target sequences of aspergillus fumigatus mitochondrion gene. And the PCR reaction system was established by preliminary verification of its performances. Results The PCR method established had good sensitivity,stability and specificity with a detection linear range of from 10 super(2) to 10 super(8) copies/mL. Conclusion The PCR method established have good performances. But the clinical use of this method needs to be verified by more multi-center research.