Strong suppression of chloramphenicol acetyltransferase expression by inducing T7 RNA polymerase

Strong suppression of chloramphenicol acetyltransferase expression by inducing T7 RNA polymerase

Suppression of gene expression can be used as a way to study gene regulation. Expression of chloramphenicol acetyltransferase (CAT) under E. coli promoter was inhibited by 92% by induction of T7 RNA polymerase which transcribes from a T7 promoter opposing E. coli promoter.

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Bibliographische Detailangaben
Veröffentlicht in:Biotechnology letters 1991-10, Vol.13 (10), p.751-754
Hauptverfasser: Kim, Han Bok, Kang, Changwon
Format: Artikel
Sprache:eng
Schlagworte:
Bacteriology
Biological and medical sciences
chloramphenicol acetyltransferase
DNA-directed RNA polymerase
Fundamental and applied biological sciences. Psychology
gene expression
genes
Genetics
Microbiology
phage T7
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Zusammenfassung:Suppression of gene expression can be used as a way to study gene regulation. Expression of chloramphenicol acetyltransferase (CAT) under E. coli promoter was inhibited by 92% by induction of T7 RNA polymerase which transcribes from a T7 promoter opposing E. coli promoter.
ISSN:0141-5492
1573-6776
DOI:10.1007/BF01088181