A single base change in the acceptor stem of tRNA super(Leu) confers resistance upon Escherichia coli to the calmodulin inhibitor, 48/80

A single base change in the acceptor stem of tRNA super(Leu) confers resistance upon Escherichia coli to the calmodulin inhibitor, 48/80

We have isolated several classes of spontaneous mutants resistant to the calmodulin inhibitor 48/80 which inhibits cell division in Escherichia coli K12. By subcloning, complementation analysis and sequencing, the feeB locus was identified as identical to the tRNA super(L) sub(C) super(e) sub(U) sup...

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Veröffentlicht in:The EMBO journal 1991-01, Vol.10 (10), p.3113-3122
Hauptverfasser: Chen, M X, Bouquin, N, Norris, V, Casaregola, S, Seror, S J, Holland, IB
Format: Artikel
Sprache:eng
Schlagworte:
Escherichia coli
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Zusammenfassung:We have isolated several classes of spontaneous mutants resistant to the calmodulin inhibitor 48/80 which inhibits cell division in Escherichia coli K12. By subcloning, complementation analysis and sequencing, the feeB locus was identified as identical to the tRNA super(L) sub(C) super(e) sub(U) super(u) sub(A) gene. We discuss the possibility that the mutant tRNA super(L) sub(3) super(e) super(u) is limiting for the synthesis of a polypeptide(s).
ISSN:0261-4189