Purification of human leucocyte DNA: Proteinase K is not necessary

A rapid nontoxic method for the purification of DNA from human leucocytes is described. Preliminary experiments which tested different methods of DNA purification indicated that digestion of proteins with proteinase K was unnecessary. This led to the development of a simple procedure involving lysis...

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Veröffentlicht in:	Analytical biochemistry 1992-03, Vol.201 (2), p.362-365
Hauptverfasser:	Douglas, Andrea M., Georgalis, Anna M., Benton, Linda R., Canavan, Karen L., Atchison, Bentley A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Diverse techniques DNA - isolation & purification Endopeptidase K Fundamental and applied biological sciences. Psychology Humans Leukocytes - chemistry Molecular and cellular biology Nucleic Acid Hybridization proteinase K Serine Endopeptidases
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Beschreibung
Zusammenfassung:	A rapid nontoxic method for the purification of DNA from human leucocytes is described. Preliminary experiments which tested different methods of DNA purification indicated that digestion of proteins with proteinase K was unnecessary. This led to the development of a simple procedure involving lysis of the cells in SDS followed by extraction with 6 m NaCl. The method described overcomes the requirement for lengthy incubations in the presence of expensive proteinase K and subsequent extraction with toxic chemicals.
ISSN:	0003-2697 1096-0309
DOI:	10.1016/0003-2697(92)90352-8