ATP regeneration by cyclic photophosphorylation using spinach thylakoid

ATP regeneration by cyclic photophosphorylation using spinach thylakoid

ATP photophosphorylation by spinach thylakoid was examined to evaluate its use as an ATP regeneration reaction in biosynthetic reactors that consume ATP. Initial rate of cyclic photophosphorylation mediated by phenazine methosulfate was found to be 218 micromole ATP/h.mg Chlorophyll. This activity w...

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Veröffentlicht in:Biotechnology letters 1991-06, Vol.13 (6), p.411-416
Hauptverfasser: Yu, A.H.C, Hosono, K
Format: Artikel
Sprache:eng
Schlagworte:
adenosine triphosphate
ATP
bioreactors
immobilization
kinases
membranes
photophosphorylation
Spinacia oleracea
stability
storage
temperature
thylakoids
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Zusammenfassung:ATP photophosphorylation by spinach thylakoid was examined to evaluate its use as an ATP regeneration reaction in biosynthetic reactors that consume ATP. Initial rate of cyclic photophosphorylation mediated by phenazine methosulfate was found to be 218 micromole ATP/h.mg Chlorophyll. This activity was stable for over 3 months at -85 degrees C. When phosphoryl transfer reactions were coupled to cyclic photophosphorylation, ATP was continuously regenerated by thylakoid between 14-24 times in batch reactors.
ISSN:0141-5492
1573-6776
DOI:10.1007/bf01030992