Transport characteristics of crustacean hepatopancreatic and antennal gland epithelia in primary cell culture

Transport characteristics of hepatopancreatic and antennal gland epithelial cells in the lobster (Homarus americanus ), the freshwater prawn (Macrobrachium rosenbergii ), and the crayfish (Procambrus clarkii ) were observed in vitro with cells maintained on a permeable filter support. This study investigated the electrical properties of the tissues by measuring the electrical resistance and potential difference (PD) developed across the cell layer over time. Preliminary data indicate that crustacean epithelial monolayers may be utilized to study transcellular nutrient transport.