Fractal dimensions of suspended solids in streams: comparison of sampling and analysis techniques

Fluvial suspended sediment typically consists of a variety of complex, composite particles referred to as flocs. Floc characteristics are determined by factors such as the source, size and geochemical properties of the primary particles, chemical and biological coagulation processes in the water column and shear stress and turbulence levels in the stream. Studies of floc morphology have used two contrasting methods of sampling and analysis. In the first method, particles settle on a microscope slide and are observed from below using an inverted microscope. The second method uses filtration at no or low vacuum and particles deposited on the filter are observed with a microscope. Floc morphology can be quantified using fractal dimensions. The aims of the present study were to examine the effect of the two sampling methods on the fractal dimensions of particle populations, and to evaluate for each method how well the fractal dimensions at the various sampling sites reflect basin conditions. Suspended solids were collected in triplicate on inverted microscope slides and on 0·45 μm Millipore HA filters in two southern Ontario streams with contrasting riparian zones during a minor runoff event resulting from the melt of a freshly fallen snowpack. An image analysis system was used to determine area, longest axis and perimeter of particles. The morphology of the particle population of each sample was characterized using four fractal dimensions (D, D1, D2 and DK). Systematic differences in fractal dimensions obtained with the two methods were observed. For the settling method, outlines of larger particles were frequently blurred because of the distance between the focal plane (the top of the inverted microscope slides) and the plane of the particle outline. In this method, the blurring of large particles can cause an increase in the projected area and length of the particle. The effect on the particle perimeter is unpredictable because it depends on the amount of detail lost through blurring and its effect on the apparent increase in particle size. Because of blurring, D and D1 tend to be systematically lower for the settling method, whereas the net effect on D2 is unpredictable. Particle size distributions derived from settling are typically coarser because small, low density particles may remain in the water column and all particles may not deposit on the slides. This loss of fines results in systematically lower DK values for the settling method compared with th