Direct enzyme-substrate affinity determination by real-time hyperpolarized (13)C-MRS

A specialized kinetic analysis of real-time hyperpolarized [1,1,2,2-D4, 1-(13)C]choline (13)C-magnetic resonance spectroscopy enabled the determination of initial rates of metabolic enzyme activity (choline oxidase), enzyme-substrate affinity (Km), and inhibition. In a clinical MRI scanner, metaboli...

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Veröffentlicht in:	Chemical communications (Cambridge, England) England), 2014-11, Vol.50 (89), p.13801-13804
Hauptverfasser:	Friesen-Waldner, L J, Wiens, C N, Wade, T P, Thind, K, Sinclair, K P, Hovav, Y, Gomori, J M, Sosna, J, McKenzie, C A, Katz-Brull, R
Format:	Artikel
Sprache:	eng
Schlagworte:	Alcohol Oxidoreductases - chemistry Choline - chemistry Magnetic Resonance Spectroscopy
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	A specialized kinetic analysis of real-time hyperpolarized [1,1,2,2-D4, 1-(13)C]choline (13)C-magnetic resonance spectroscopy enabled the determination of initial rates of metabolic enzyme activity (choline oxidase), enzyme-substrate affinity (Km), and inhibition. In a clinical MRI scanner, metabolite levels lower than 16 μM were detected at a temporal resolution of 1 s.
ISSN:	1364-548X
DOI:	10.1039/c4cc05418k