Cloning and expression in Escherichia coli of tryptophan genes from Streptomyces griseus IMRU 3570

The Sau3A fragments of Streptomyces grisues IMRU 3750 were cloned in pBR322 as a vector. One of these clones contained the genetic information needed to complement trpA and trpB mutations in Escherichia coli. The other complements trpA, trpB and trpC mutations in E. coli. Both fragments originated i...

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Veröffentlicht in:	FEMS microbiology letters 1990-03, Vol.68 (1‐2), p.201-205
Hauptverfasser:	Rivero‐Lezcano, Octavio, Anguita‐Castillo, Juan, López‐Nieto, J., Naharro‐Carrasco, Germán
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteriology Biological and medical sciences Blotting, Southern Cloning Cloning, Molecular DNA DNA, Bacterial - genetics Escherichia coli Escherichia coli - genetics Expression Fundamental and applied biological sciences. Psychology Gene Expression Genes, Bacterial Genetic Complementation Test Genetics Microbiology Restriction Mapping Streptomyces griseus - genetics tryptophan Tryptophan - biosynthesis Tryptophan - genetics Tryptophan genes
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Zusammenfassung:	The Sau3A fragments of Streptomyces grisues IMRU 3750 were cloned in pBR322 as a vector. One of these clones contained the genetic information needed to complement trpA and trpB mutations in Escherichia coli. The other complements trpA, trpB and trpC mutations in E. coli. Both fragments originated in the same region of the chromosome but the latter is 1 kilobase (kb) longer in the region nearest the tetracycline promoter.
ISSN:	0378-1097 1574-6968
DOI:	10.1111/j.1574-6968.1990.tb04149.x