A newly isolated Bacillus stearothermophilus K1041 and its transformation by electroporation

Optimal conditions for the plasmid transformation of a newly isolated Bacillus stearothermophilus K1041 by electroporation were investigated. The optimal conditions allowed a transformation efficiency of 5.8 x 10 super(5) transformants per mu g plasmid pUB10.

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Bibliographische Detailangaben
Veröffentlicht in:	Biotechnology techniques 1992, Vol.6 (1), p.83-86
Hauptverfasser:	NARUMI, I, SAWAKAMI, K, NAKAMOTO, S, NAKAYAMA, N, YANAGISAWA, T, TAKAHASHI, N, KIHARA, H
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology DNA electroporation Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Methods. Procedures. Technologies Miscellaneous plasmids Synthetic digonucleotides and genes. Sequencing transformation
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Beschreibung
Zusammenfassung:	Optimal conditions for the plasmid transformation of a newly isolated Bacillus stearothermophilus K1041 by electroporation were investigated. The optimal conditions allowed a transformation efficiency of 5.8 x 10 super(5) transformants per mu g plasmid pUB10.
ISSN:	0951-208X 1573-6784
DOI:	10.1007/BF02438695