Investigation of glycohemoglobin A1c detection status in Zhongshan, Guangdong

Objective To investigate the status of glycohemoglobin A1c detection in Zhongshan area. Methods Bio-Rad VARIANT II TURBO glycohemoglobin analyzer was chosen as a comparison system, Bio-Rad D-10 glycohemoglobin analyzer which through the NGSP certification, was chosen as a reference system. 20 different concentrations of fresh whole blood was collected for comparison. Then through comparison system on two fresh whole blood assignment, the two assignment of fresh whole blood and the other three valued Bio-Rad global chamber investigation were surveyed to 17 laboratories. The results were statistically analyzed. Results The comparison system and reference system were comparable. In 17 laboratories used 12 sets of detection system, and the analysis methods included ion exchange high performance liquid chromatography (IE-HPLC), immunoturbidimetry and particulate chromatography. Detection of glycohemoglobin A1c percentage bias range and coefficient of variation (CV) range were 0.00%-32.29% and 2.30%-14.59% respecti