Preparation and evaluation of quality control serum of autoantibodies threshold

Objective To investigate and evaluate the preparation of quality control serum of autoantibodies threshold. Methods Donors' serum blood were placed in 56 [degrees]C for 30 min to inactivate, followed by centrifuged to remove the precipitates, and then multi-batch quality control material in detection kit were detected in dilution ratios of 1 : 2, 1 : 4, 1 : 6. Results Anti-RNP/ Sm antibody(+ + +): after 1 : 2 dilution, the coloration degree was + + +; after 1 : 4, 1 : 6 dilution, the coloration degree was + +. Anti-SS-A antibody (+ + to + + +): after 1 : 2 dilution, the coloration degree was + +; after 1 : 4, 1 : 6 dilution, the coloration degree was + to + +, Anti-RO-52 antibody(+ + to + + +): after 1 : 2 dilution, the coloration degree was + to + +; after 1 : 4, 1 : 6 dilution, the coloration degree was +. Anti-nucleosome antibodies: positive were found in dilution of 1 : 1, 1 : 2; after 1 : 4, 1 : 6 dilution, the coloration was negative. Conclusion Preparation of quality control serum of autoantibodies is