Sequence-specific affinity selection of mammalian splicing complexes

Sequence-specific affinity selection of mammalian splicing complexes

Antisense oligonucleotides made of 2'-OMe RNA are shown to bind specifically and efficiently to targeted sites on pre-mRNA substrates, allowing affinity selection of splicing complexes using streptavidin/biotin chromatography. Quantitative affinity selection analyses show that only a small frac...

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Veröffentlicht in:Nucleic acids research 1991-01, Vol.18 (24), p.7373-7379
Hauptverfasser: Ryder, U, Sproat, B S, Lamond, AI
Format: Artikel
Sprache:eng
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Zusammenfassung:Antisense oligonucleotides made of 2'-OMe RNA are shown to bind specifically and efficiently to targeted sites on pre-mRNA substrates, allowing affinity selection of splicing complexes using streptavidin/biotin chromatography. Quantitative affinity selection analyses show that only a small fraction of snRNPs in a HeLa nuclear splicing extract participate in spliceosome formation.
ISSN:0305-1048