Development and optimization of a defined medium for aerobic growth of Bacillus stearothermophilus LLD-15

Development and optimization of a defined medium for aerobic growth of Bacillus stearothermophilus LLD-15

Methionine, biotin, nicotinic acid and thiamine were found to be absolute requirements for growth of Bacillus stearothermophilus strain LLD-15, an L-lactate dehydrogenase mutant, at 70 degree C. A defined medium for aerobic growth did not support anaerobic growth. Physiological and subsequent taxono...

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Veröffentlicht in:Biotechnology letters 1991-09, Vol.13 (9), p.621-626
Hauptverfasser: AMARTEY, S. A, LEAK, D. J, HARTLEY, B. S
Format: Artikel
Sprache:eng
Schlagworte:
Bacteriology
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Microbiology
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Zusammenfassung:Methionine, biotin, nicotinic acid and thiamine were found to be absolute requirements for growth of Bacillus stearothermophilus strain LLD-15, an L-lactate dehydrogenase mutant, at 70 degree C. A defined medium for aerobic growth did not support anaerobic growth. Physiological and subsequent taxonomic studies showed that strain LLD-15 is not a derivative of B. stearothermophilus NCA 1503 as originally thought, but bears some similarity to B. caldotenax .
ISSN:0141-5492
1573-6776
DOI:10.1007/BF01086315