Transgenic sugarcane plants resistant to stem borer attack

Transgenic sugarcane plants resistant to stem borer attack

A truncated cryIA(b) gene encoding the active region of the Bacillus thuringiensis δ-endotoxin was expressed in transgenic sugarcane plants (Saccharum officinarum L.) under the control of the CaMV 35S promoter. Genetic transformation was accomplished by electroporation of intact cells. The levels of...

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Veröffentlicht in:Molecular breeding 1997-08, Vol.3 (4), p.247-255
Hauptverfasser: Arencibia, A. (Center for Genetic Engineering and Biotechnology, Havana (Cuba). Plant Div.), Vazquez, R.I, Prieto, D, Tellez, P, Carmona, E.R, Coego, A, Hernandez, L, Riva, G.A. de la, Selman-Housein, G
Format: Artikel
Sprache:eng
Schlagworte:
BACILLUS THURINGIENSIS
Biological activity
Borers
Diatraea saccharalis
Electroporation
ENDOTOXINAS
ENDOTOXINE
ENDOTOXINS
EXPRESION GENICA
EXPRESSION DES GENES
GENE EXPRESSION
Genetic transformation
Larvae
Larvicides
Molecular biology
PEST RESISTANCE
Plant biology
PLANTAS TRANSGENICAS
PLANTE TRANSGENIQUE
RESISTANCE AUX ORGANISMES NUISIBLES
RESISTENCIA A LAS PLAGAS
SACCHARUM OFFICINARUM
Sugarcane
Toxins
TRANSGENIC PLANTS
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Beschreibung
Zusammenfassung:A truncated cryIA(b) gene encoding the active region of the Bacillus thuringiensis δ-endotoxin was expressed in transgenic sugarcane plants (Saccharum officinarum L.) under the control of the CaMV 35S promoter. Genetic transformation was accomplished by electroporation of intact cells. The levels of recombinant toxin were established and biological activity tests were performed against neonate sugarcane borer (Diatraea saccharalis F.) larvae. Transgenic sugarcane plants showed significant larvicidal activity despite the low expression of CryIA(b).
ISSN:1380-3743
1572-9788
DOI:10.1023/A:1009616318854