Site-directed Mutagenesis Applied to Glucose Isomerase from Streptomyces violaceoniger and Streptomyces olivochromogenes

Site-directed Mutagenesis Applied to Glucose Isomerase from Streptomyces violaceoniger and Streptomyces olivochromogenes

Two xylose isomerases industrially used in France are produced from Streptomyces olivochromogenes ATCC 21713 and Streptomyces violaceoniger CBS 409-73. In order to improve the catalytic efficiency of the enzyme and its operational stability, two approaches have been taken: the first consisted of a s...

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Veröffentlicht in:Annals of the New York Academy of Sciences 1990-01, Vol.613 (1), p.371-375
Hauptverfasser: SICARD, P. J., LELEU, J-B., DUFLOT, P., DROCOURT, D., MARTI, F., TIRABY, G., PETSKO, G., GLASFELD, A.
Format: Artikel
Sprache:eng
Schlagworte:
Aldose-Ketose Isomerases
Amino Acid Sequence
Carbohydrate Epimerases - genetics
Carbohydrate Epimerases - metabolism
Enzyme Stability
Escherichia coli - genetics
genes
Hot Temperature
Molecular Sequence Data
Mutagenesis, Site-Directed
Plasmids
Restriction Mapping
site-directed mutagenesis
Streptomyces - enzymology
Streptomyces - genetics
Streptomyces olivochromogenes
xylose isomerase
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Zusammenfassung:Two xylose isomerases industrially used in France are produced from Streptomyces olivochromogenes ATCC 21713 and Streptomyces violaceoniger CBS 409-73. In order to improve the catalytic efficiency of the enzyme and its operational stability, two approaches have been taken: the first consisted of a site-directed mutagenesis of a residue essential for thermostability, whereas the other consisted of a modification of the whole protein through gene manipulation in E. coli .
ISSN:0077-8923
1749-6632
DOI:10.1111/j.1749-6632.1990.tb18181.x