Fast nuclear staining of head hair roots as a screening method for successful STR analysis in forensics

Fast nuclear staining of head hair roots as a screening method for successful STR analysis in forensics

Highlights • Two DAPI staining methods to stain nuclear DNA in head hair roots are compared. • Nuclear DNA in head hair roots can be directly visualized after staining. • Minimum 20 nuclei are required to obtain at least partial DNA profiles. • Adhesive tapes to collect hairs have no influence on th...

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Veröffentlicht in:Forensic science international : genetics 2014-11, Vol.13, p.191-194
Hauptverfasser: Lepez, Trees, Vandewoestyne, Mado, Van Hoofstat, David, Deforce, Dieter
Format: Artikel
Sprache:eng
Schlagworte:
Cell Nucleus
DNA Fingerprinting - methods
Fluorescent Dyes
Hair - cytology
Hair - ultrastructure
Humans
Indoles
Microsatellite Repeats
Microscopy, Fluorescence
Pathology
Staining and Labeling
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Zusammenfassung:Highlights • Two DAPI staining methods to stain nuclear DNA in head hair roots are compared. • Nuclear DNA in head hair roots can be directly visualized after staining. • Minimum 20 nuclei are required to obtain at least partial DNA profiles. • Adhesive tapes to collect hairs have no influence on the DAPI staining. • This screening method can be used to select hairs suitable for DNA analysis.
ISSN:1872-4973
1878-0326
DOI:10.1016/j.fsigen.2014.08.003