DNA Fingerprinting with Oligonucleotides Can Differentiate Cell Lines Derived from the Same Tumor
Oligonucleotide fingerprinting was applied to investigate the relatedness of several cell lines that were established between 1973 and 1977 from a teratocarcinoma. We were able to distinguish cell lines derived at different times. In addition, sublines from one cell line (PYS-2) could be discriminat...
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| Veröffentlicht in: | In Vitro Cellular & Developmental Biology - Animal 1991-08, Vol.27A (8), p.646-650 |
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| container_end_page | 650 |
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| container_issue | 8 |
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| container_title | In Vitro Cellular & Developmental Biology - Animal |
| container_volume | 27A |
| creator | Cornelia Speth Epplen, Jörg T. Ilse Oberbäumer |
| description | Oligonucleotide fingerprinting was applied to investigate the relatedness of several cell lines that were established between 1973 and 1977 from a teratocarcinoma. We were able to distinguish cell lines derived at different times. In addition, sublines from one cell line (PYS-2) could be discriminated by using a combination of different probes. Therefore multilocus fingerprinting with oligonucleotides is a useful method for monitoring changes in cell lines kept in culture for many generations. |
| doi_str_mv | 10.1007/BF02631108 |
| format | Article |
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We were able to distinguish cell lines derived at different times. In addition, sublines from one cell line (PYS-2) could be discriminated by using a combination of different probes. Therefore multilocus fingerprinting with oligonucleotides is a useful method for monitoring changes in cell lines kept in culture for many generations.</description><identifier>ISSN: 0883-8364</identifier><identifier>EISSN: 2327-431X</identifier><identifier>EISSN: 1543-706X</identifier><identifier>DOI: 10.1007/BF02631108</identifier><identifier>PMID: 1917782</identifier><identifier>CODEN: ICDBEO</identifier><language>eng</language><publisher>Largo, MD: Tissue Culture Association, Inc</publisher><subject>Animals ; Base Sequence ; Biological and medical sciences ; Cell Differentiation ; Cell Line ; Cell lines ; DNA ; DNA - isolation & purification ; DNA Fingerprinting ; DNA probes ; DNA, Neoplasm - isolation & purification ; Fundamental and applied biological sciences. 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Biological and molecular evolution ; Mice ; Molecular Sequence Data ; Neurons ; Oligonucleotide Probes ; Pluripotent stem cells ; Stem cells ; Teratoma - genetics ; Teratoma - pathology ; Time Factors ; Tumor cell line ; Tumor stem cells ; Tumors</subject><ispartof>In Vitro Cellular & Developmental Biology - Animal, 1991-08, Vol.27A (8), p.646-650</ispartof><rights>Copyright 1991 Tissue Culture Association</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c363t-d52b5c58a78ed1e02f3ad2de2a327d561903fbb5e2c389acb9d7f632f3d745fa3</citedby><cites>FETCH-LOGICAL-c363t-d52b5c58a78ed1e02f3ad2de2a327d561903fbb5e2c389acb9d7f632f3d745fa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4296721$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4296721$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5261979$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1917782$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cornelia Speth</creatorcontrib><creatorcontrib>Epplen, Jörg T.</creatorcontrib><creatorcontrib>Ilse Oberbäumer</creatorcontrib><title>DNA Fingerprinting with Oligonucleotides Can Differentiate Cell Lines Derived from the Same Tumor</title><title>In Vitro Cellular & Developmental Biology - Animal</title><addtitle>In Vitro Cell Dev Biol</addtitle><description>Oligonucleotide fingerprinting was applied to investigate the relatedness of several cell lines that were established between 1973 and 1977 from a teratocarcinoma. We were able to distinguish cell lines derived at different times. In addition, sublines from one cell line (PYS-2) could be discriminated by using a combination of different probes. Therefore multilocus fingerprinting with oligonucleotides is a useful method for monitoring changes in cell lines kept in culture for many generations.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cell Differentiation</subject><subject>Cell Line</subject><subject>Cell lines</subject><subject>DNA</subject><subject>DNA - isolation & purification</subject><subject>DNA Fingerprinting</subject><subject>DNA probes</subject><subject>DNA, Neoplasm - isolation & purification</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gels</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Neurons</subject><subject>Oligonucleotide Probes</subject><subject>Pluripotent stem cells</subject><subject>Stem cells</subject><subject>Teratoma - genetics</subject><subject>Teratoma - pathology</subject><subject>Time Factors</subject><subject>Tumor cell line</subject><subject>Tumor stem cells</subject><subject>Tumors</subject><issn>0883-8364</issn><issn>2327-431X</issn><issn>1543-706X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkM1LAzEUxIMotVYvnhVyEA_Caj66m-yxtlaFogcreFuyyYum7EdNdhX_eyMt9fQezI9hZhA6peSaEiJubueEZZxSIvfQkHEmkjGnb_toSKTkieTZ-BAdhbAihJOMsQEa0JwKIdkQqdnTBM9d8w5-7V3TxQ9_u-4DP1fuvW16XUHbOQMBT1WDZ85a8BAx1QGeQlXhhWuiOAPvvsBg69sadx-AX1QNeNnXrT9GB1ZVAU62d4Re53fL6UOyeL5_nE4WieYZ7xKTsjLVqVRCgqFAmOXKMANMxT4mzWhOuC3LFJjmMle6zI2wGY-YEePUKj5ClxvftW8_ewhdUbugY0TVQNuHgmZESBInGKGrDah9G4IHW8TmtfI_BSXF357F_54RPt-69mUN5h_dDBj1i62uglaV9arRLuywlMXgIo_Y2QZbha71O3nM8kwwyn8BqUqGJg</recordid><startdate>19910801</startdate><enddate>19910801</enddate><creator>Cornelia Speth</creator><creator>Epplen, Jörg T.</creator><creator>Ilse Oberbäumer</creator><general>Tissue Culture Association, Inc</general><general>Society for In Vitro Biology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope></search><sort><creationdate>19910801</creationdate><title>DNA Fingerprinting with Oligonucleotides Can Differentiate Cell Lines Derived from the Same Tumor</title><author>Cornelia Speth ; Epplen, Jörg T. ; Ilse Oberbäumer</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c363t-d52b5c58a78ed1e02f3ad2de2a327d561903fbb5e2c389acb9d7f632f3d745fa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cell Differentiation</topic><topic>Cell Line</topic><topic>Cell lines</topic><topic>DNA</topic><topic>DNA - isolation & purification</topic><topic>DNA Fingerprinting</topic><topic>DNA probes</topic><topic>DNA, Neoplasm - isolation & purification</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gels</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>Neurons</topic><topic>Oligonucleotide Probes</topic><topic>Pluripotent stem cells</topic><topic>Stem cells</topic><topic>Teratoma - genetics</topic><topic>Teratoma - pathology</topic><topic>Time Factors</topic><topic>Tumor cell line</topic><topic>Tumor stem cells</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cornelia Speth</creatorcontrib><creatorcontrib>Epplen, Jörg T.</creatorcontrib><creatorcontrib>Ilse Oberbäumer</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><jtitle>In Vitro Cellular & Developmental Biology - Animal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cornelia Speth</au><au>Epplen, Jörg T.</au><au>Ilse Oberbäumer</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DNA Fingerprinting with Oligonucleotides Can Differentiate Cell Lines Derived from the Same Tumor</atitle><jtitle>In Vitro Cellular & Developmental Biology - Animal</jtitle><addtitle>In Vitro Cell Dev Biol</addtitle><date>1991-08-01</date><risdate>1991</risdate><volume>27A</volume><issue>8</issue><spage>646</spage><epage>650</epage><pages>646-650</pages><issn>0883-8364</issn><eissn>2327-431X</eissn><eissn>1543-706X</eissn><coden>ICDBEO</coden><abstract>Oligonucleotide fingerprinting was applied to investigate the relatedness of several cell lines that were established between 1973 and 1977 from a teratocarcinoma. We were able to distinguish cell lines derived at different times. In addition, sublines from one cell line (PYS-2) could be discriminated by using a combination of different probes. Therefore multilocus fingerprinting with oligonucleotides is a useful method for monitoring changes in cell lines kept in culture for many generations.</abstract><cop>Largo, MD</cop><pub>Tissue Culture Association, Inc</pub><pmid>1917782</pmid><doi>10.1007/BF02631108</doi><tpages>5</tpages></addata></record> |
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| identifier | ISSN: 0883-8364 |
| ispartof | In Vitro Cellular & Developmental Biology - Animal, 1991-08, Vol.27A (8), p.646-650 |
| issn | 0883-8364 2327-431X 1543-706X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_16078000 |
| source | Jstor Complete Legacy; MEDLINE; SpringerLink Journals |
| subjects | Animals Base Sequence Biological and medical sciences Cell Differentiation Cell Line Cell lines DNA DNA - isolation & purification DNA Fingerprinting DNA probes DNA, Neoplasm - isolation & purification Fundamental and applied biological sciences. Psychology Gels Genetics of eukaryotes. Biological and molecular evolution Mice Molecular Sequence Data Neurons Oligonucleotide Probes Pluripotent stem cells Stem cells Teratoma - genetics Teratoma - pathology Time Factors Tumor cell line Tumor stem cells Tumors |
| title | DNA Fingerprinting with Oligonucleotides Can Differentiate Cell Lines Derived from the Same Tumor |
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