DNA Fingerprinting with Oligonucleotides Can Differentiate Cell Lines Derived from the Same Tumor

Oligonucleotide fingerprinting was applied to investigate the relatedness of several cell lines that were established between 1973 and 1977 from a teratocarcinoma. We were able to distinguish cell lines derived at different times. In addition, sublines from one cell line (PYS-2) could be discriminat...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	In Vitro Cellular & Developmental Biology - Animal 1991-08, Vol.27A (8), p.646-650
Hauptverfasser:	Cornelia Speth, Epplen, Jörg T., Ilse Oberbäumer
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence Biological and medical sciences Cell Differentiation Cell Line Cell lines DNA DNA - isolation & purification DNA Fingerprinting DNA probes DNA, Neoplasm - isolation & purification Fundamental and applied biological sciences. Psychology Gels Genetics of eukaryotes. Biological and molecular evolution Mice Molecular Sequence Data Neurons Oligonucleotide Probes Pluripotent stem cells Stem cells Teratoma - genetics Teratoma - pathology Time Factors Tumor cell line Tumor stem cells Tumors
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Oligonucleotide fingerprinting was applied to investigate the relatedness of several cell lines that were established between 1973 and 1977 from a teratocarcinoma. We were able to distinguish cell lines derived at different times. In addition, sublines from one cell line (PYS-2) could be discriminated by using a combination of different probes. Therefore multilocus fingerprinting with oligonucleotides is a useful method for monitoring changes in cell lines kept in culture for many generations.
ISSN:	0883-8364 2327-431X 1543-706X
DOI:	10.1007/BF02631108