Evidence of Superoxide Dismutase in Lactobacillus acidophilus
Superoxide dismutase was prepared from Lactobacillus acidophilus CRL 358. An approximately 191-fold increase in the specific activity of the enzyme was achieved. The extract purified to apparent homogenity and exhibited a single band following polyacrylamide gel electrophoresis which detected supero...
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Veröffentlicht in: | Chemical & pharmaceutical bulletin 1991/04/25, Vol.39(4), pp.1065-1067 |
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container_title | Chemical & pharmaceutical bulletin |
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creator | GONZALEZ, Silvia N. CHAUD, Carlos A. NADRA APELLA, Maria C. Ana M. STRASSER DE SAAD OLIVER, Guillermo |
description | Superoxide dismutase was prepared from Lactobacillus acidophilus CRL 358. An approximately 191-fold increase in the specific activity of the enzyme was achieved. The extract purified to apparent homogenity and exhibited a single band following polyacrylamide gel electrophoresis which detected superoxide dismutase activity. The apparent molecular weight determined by filtration was about 160000 dalton (Da). |
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NADRA ; APELLA, Maria C. ; Ana M. STRASSER DE SAAD ; OLIVER, Guillermo</creator><creatorcontrib>GONZALEZ, Silvia N. ; CHAUD, Carlos A. NADRA ; APELLA, Maria C. ; Ana M. STRASSER DE SAAD ; OLIVER, Guillermo</creatorcontrib><description>Superoxide dismutase was prepared from Lactobacillus acidophilus CRL 358. An approximately 191-fold increase in the specific activity of the enzyme was achieved. The extract purified to apparent homogenity and exhibited a single band following polyacrylamide gel electrophoresis which detected superoxide dismutase activity. 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The apparent molecular weight determined by filtration was about 160000 dalton (Da).</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>enzyme activity</subject><subject>Enzymes and enzyme inhibitors</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gel electrophoresis</subject><subject>Lactobacillus acidophilus</subject><subject>molecular weight</subject><subject>Oxidoreductases</subject><subject>purification</subject><subject>superoxide dismutase</subject><issn>0009-2363</issn><issn>1347-5223</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><recordid>eNpFkD1PwzAURS0EEqUw8QcyIBaU4m_HAwOi5UOqxADMlu041FWaBDtB8O9xlKosfta7553hAnCJ4AJhWtzaziyIXCDI2RGYIUJFzjAmx2AGIZQ5JpycgrMYtxBiBgWZgbvVty9dY13WVtnb0LnQ_qRFtvRxN_Q6usw32VrbvjXa-roeYpZm2XYbn_7n4KTSdXQX-zkHH4-r94fnfP369PJwv84to6jPCeOCFKiiUHMjhbGcEWYINg7KEuOUICtESh3T1ApY6lJaSbmThtGqMmQOridvF9qvwcVe7Xy0rq5149ohKsShQKwgCbyZQBvaGIOrVBf8TodfhaAaK1KpIkWkGitK9NVeq6PVdRV0Y308nFApJUFFwpYTto29_nSHXIfe29qNSiRZMWrp9Iz2_3ijg3IN-QNSwH4o</recordid><startdate>1991</startdate><enddate>1991</enddate><creator>GONZALEZ, Silvia N.</creator><creator>CHAUD, Carlos A. 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STRASSER DE SAAD</au><au>OLIVER, Guillermo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evidence of Superoxide Dismutase in Lactobacillus acidophilus</atitle><jtitle>Chemical & pharmaceutical bulletin</jtitle><addtitle>Chem. Pharm. Bull.</addtitle><date>1991</date><risdate>1991</risdate><volume>39</volume><issue>4</issue><spage>1065</spage><epage>1067</epage><pages>1065-1067</pages><issn>0009-2363</issn><eissn>1347-5223</eissn><coden>CPBTAL</coden><abstract>Superoxide dismutase was prepared from Lactobacillus acidophilus CRL 358. An approximately 191-fold increase in the specific activity of the enzyme was achieved. The extract purified to apparent homogenity and exhibited a single band following polyacrylamide gel electrophoresis which detected superoxide dismutase activity. 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subjects | Analytical, structural and metabolic biochemistry Biological and medical sciences enzyme activity Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology gel electrophoresis Lactobacillus acidophilus molecular weight Oxidoreductases purification superoxide dismutase |
title | Evidence of Superoxide Dismutase in Lactobacillus acidophilus |
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