Evidence of Superoxide Dismutase in Lactobacillus acidophilus

Evidence of Superoxide Dismutase in Lactobacillus acidophilus

Superoxide dismutase was prepared from Lactobacillus acidophilus CRL 358. An approximately 191-fold increase in the specific activity of the enzyme was achieved. The extract purified to apparent homogenity and exhibited a single band following polyacrylamide gel electrophoresis which detected supero...

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Veröffentlicht in:Chemical & pharmaceutical bulletin 1991/04/25, Vol.39(4), pp.1065-1067
Hauptverfasser: GONZALEZ, Silvia N., CHAUD, Carlos A. NADRA, APELLA, Maria C., Ana M. STRASSER DE SAAD, OLIVER, Guillermo
Format: Artikel
Sprache:eng
Schlagworte:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
enzyme activity
Enzymes and enzyme inhibitors
Fundamental and applied biological sciences. Psychology
gel electrophoresis
Lactobacillus acidophilus
molecular weight
Oxidoreductases
purification
superoxide dismutase
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Beschreibung
Zusammenfassung:Superoxide dismutase was prepared from Lactobacillus acidophilus CRL 358. An approximately 191-fold increase in the specific activity of the enzyme was achieved. The extract purified to apparent homogenity and exhibited a single band following polyacrylamide gel electrophoresis which detected superoxide dismutase activity. The apparent molecular weight determined by filtration was about 160000 dalton (Da).
ISSN:0009-2363
1347-5223
DOI:10.1248/cpb.39.1065