Evaluation of commercial soybean inoculants from Argentina

Eighteen samples of soybean inoculants representative of the major manufacturing companies in Argentina were purchased from the market and evaluated using plate counts, most probable number (MPN) of Bradyrhizobium japonicum on plants and time of nodule appearance. One or two B. japonicum isolates per product were isolated and typed by analysis of their DNA patterns. The Log10 numbers of B. japonicum obtained were in the range of 0 to 6/soybean seed, with only two products above 1 × 106 bacteria/seed. Of 18 products, 17 were contaminated, and of these 14 contained more contaminants than B. japonicum. The time of nodule appearance varied between 8 and 16 days, indicating a great difference in microbial activity between products. The strains were found to be similar to USDA 138 (five isolates), E45-INTA Argentina (two isolates), USDA 142 (four isolates) and E4-INTA (one isolate). Thus, even if most of the typed strains are considered as good N2-fixing strains, the average quality of the analysed samples was low, and could not support efficient inoculation of soybean.[PUBLICATION ABSTRACT]