Primary cell culture from explants of heart tissue of Indian major carps

Primary cell culture from explants of heart tissue of Indian major carps

Heart tissue of Indian major carps was explanted in minimum essential medium supplemented with 15% fetal bovine serum. Fibroblast-like cells emerged from the explant and formed a monolayer within a week. The cell monolayer could be harvested for passage by either trypsinization or scraping.

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Bibliographische Detailangaben
Veröffentlicht in:Current science (Bangalore) 1997-08, Vol.73 (4), p.374-376
Hauptverfasser: Rao, K. Sushmitha, Joseph, M. A., Shankar, K. M., Mohan, C. V.
Format: Artikel
Sprache:eng
Schlagworte:
Carp
Catla catla
Cell lines
Cirrhinus mrigala
Fetus
Fish
Fish culture
Flasks
Indian culture
Labeo rohita
Microelectromechanical systems
Primary cell culture
RESEARCH COMMUNICATIONS
Tissue culture techniques
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Zusammenfassung:Heart tissue of Indian major carps was explanted in minimum essential medium supplemented with 15% fetal bovine serum. Fibroblast-like cells emerged from the explant and formed a monolayer within a week. The cell monolayer could be harvested for passage by either trypsinization or scraping.
ISSN:0011-3891