Arylamine spider toxins antagonize NMDA receptor-mediated synaptic transmission in rat hippocampal slices

Arylamine spider toxins antagonize NMDA receptor-mediated synaptic transmission in rat hippocampal slices

The effects of arylamine spider toxins on synaptic transmission in rat hippocampal slices were investigated. Two different responses were monitored: the AMPA receptor‐mediated population spike recorded in control buffer (selectively antagonized by DNQX) and the NMDA receptor‐mediated EPSP recorded i...

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Veröffentlicht in:Synapse (New York, N.Y.) N.Y.), 1991-12, Vol.9 (4), p.244-250
Hauptverfasser: Mueller, Alan L., Albensi, Benedict C., Ganong, Alan H., Reynolds, Linda S., Jackson, Hunter
Format: Artikel
Sprache:eng
Schlagworte:
AMPA
Animals
Argiotoxins
Glutamate receptors
Hippocampus - physiology
In Vitro Techniques
Indoleacetic Acids - pharmacology
Male
Neurotoxins - pharmacology
Phenylacetates - pharmacology
Polyamines - pharmacology
Rats
Rats, Inbred Strains
Receptors, AMPA
Receptors, N-Methyl-D-Aspartate - antagonists & inhibitors
Receptors, N-Methyl-D-Aspartate - physiology
Receptors, Neurotransmitter - physiology
Spider Venoms - pharmacology
Synapses - physiology
Synaptic Transmission - drug effects
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Zusammenfassung:The effects of arylamine spider toxins on synaptic transmission in rat hippocampal slices were investigated. Two different responses were monitored: the AMPA receptor‐mediated population spike recorded in control buffer (selectively antagonized by DNQX) and the NMDA receptor‐mediated EPSP recorded in nominally magnesium‐free buffer containing 20 μM DNQX (selectively antagonized by AP5, AP7, and dizocilpine (MK‐801)). The synthetic arylamine spider toxins JSTX‐3, argiotoxin‐636, and argiotoxin‐659 were 26 to 73 times more potent at antagonizing the NMDA receptor‐mediated EPSP (IC50 values ranging from 12 to 24 μM) than the AMPA receptor‐mediated population spike (IC50 values ranging from 612 to 878 μM). These results indicate that arylamine spider toxins are selective antagonists of NMDA receptors in the mammalian CNS.
ISSN:0887-4476
1098-2396
DOI:10.1002/syn.890090403