Development of an acute and chronic ecotoxicity assay using lux‐marked Rhizobium leguminosarum biovar trifolii

A soil isolate of Rhizobium leguminosarum bv. trifolii was marked with a lux CDABE gene cassette to enable the expression of bioluminescence. The suitability of the bacterium as a soil pollution biosensor was assessed using acute and chronic assays. Bacterial bioluminescence responded sensitively to...

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Veröffentlicht in:	Letters in applied microbiology 1997-04, Vol.24 (4), p.296-300
Hauptverfasser:	Paton, G. I., Palmer, G., Burton, M., Rattray, E. A. S., McGrath, S. P., Glover, L. A., Killham, K.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal, plant and microbial ecology Applied ecology Biological and medical sciences Biosensing Techniques Biotechnology Ecotoxicology, biological effects of pollution Environment and pollution Environmental Monitoring - methods Evaluation Studies as Topic Fundamental and applied biological sciences. Psychology Genes, Bacterial Industrial applications and implications. Economical aspects Luminescent Measurements Metals - analysis Metals - toxicity Miscellaneous Rhizobium leguminosarum - drug effects Rhizobium leguminosarum - genetics Rhizobium leguminosarum trifolii Soil Pollutants - analysis Soil Pollutants - toxicity Terrestrial environment, soil, air
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Zusammenfassung:	A soil isolate of Rhizobium leguminosarum bv. trifolii was marked with a lux CDABE gene cassette to enable the expression of bioluminescence. The suitability of the bacterium as a soil pollution biosensor was assessed using acute and chronic assays. Bacterial bioluminescence responded sensitively to the metals studied. The order of sensitivity was found to be Cd > Ni = Zn > Cu for the acute test and Cd > Ni = Zn = Cu for the chronic test. The sensitive response of the biosensor highlighted its potential for use as an indicator of soil pollution.
ISSN:	0266-8254 1472-765X
DOI:	10.1046/j.1472-765X.1997.00071.x