Construction and rapid testing of synthetic and modified toxin gene sequences CryIA (b&c) by expression in maize endosperm culture

The synthesis of two modified genes, Cry IA(b) and CryIA(c), each consisting of 1845 bp, is described in detail. The genes were synthesized using an improved PCR procedure based on recursive principles. The synthetic CryIA(c) gene was put under the control of a maize ubiquitin promoter. This constru...

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Veröffentlicht in:	Plant cell reports 1996-05, Vol.15 (9), p.677-681
Hauptverfasser:	Sardana, R, Dukiandjiev, S, Giband, M, Cheng, X.Y, Cowan, K, Sauder, C, Altosaar, I
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology cell suspension culture endosperm endotoxins Fundamental and applied biological sciences. Psychology gene expression genes Genetic engineering genetic regulation Genetic technics Methods. Procedures. Technologies Modification of gene expression level polymerase chain reaction rapid methods synthesis ubiquitin Zea mays
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Zusammenfassung:	The synthesis of two modified genes, Cry IA(b) and CryIA(c), each consisting of 1845 bp, is described in detail. The genes were synthesized using an improved PCR procedure based on recursive principles. The synthetic CryIA(c) gene was put under the control of a maize ubiquitin promoter. This construct was tested in a maize endosperm-derived suspension culture system. The use of maize endosperm culture as a quick and efficient system to test the activity of synthetic genes is described.
ISSN:	0721-7714 1432-203X
DOI:	10.1007/BF00231923