Influence of cell culture conditions on the protective effect of antioxidants against β-amyloid toxicity: studies with lazaroids
The mechanisms of cell death of rat cortical neurons chronically exposed to the β-amyloid ( βA) biologically active fragment β-(25–35) involve oxidative stress. We examined the influence of culture conditions on the neuroprotective activity of antioxidants against β-(25–35) toxicity. Common radical...
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| Veröffentlicht in: | Brain research 1997-08, Vol.764 (1), p.293-298 |
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| creator | Lucca, Elisa Angeretti, Nadia Forloni, Gianluigi |
| description | The mechanisms of cell death of rat cortical neurons chronically exposed to the
β-amyloid (
βA) biologically active fragment
β-(25–35) involve oxidative stress. We examined the influence of culture conditions on the neuroprotective activity of antioxidants against
β-(25–35) toxicity. Common radical scavengers such as
N-acetylcysteine (250
μM) and
N-
t-butyl-phenylnitrone (500
μM) only protected cortical cells cultured in the presence of fetal calf serum (FCS) from
βA insult. The neuroprotective effect of lazaroids (U74389G and U83836E), 21-aminosteroids with antioxidant activity, was tested in cells grown with or without FCS. U74389G did not interfere with
β-(25–35) toxicity in either condition, while U83836E at a very low concentration (15 nM) protected cortical cells exposed to the
β peptide only when the neurons were cultured in the presence of FCS. These data show that a lazaroid can prevent
β-(25–35) toxicity and that the antioxidants exerted their protective effect in certain conditions. |
| doi_str_mv | 10.1016/S0006-8993(97)00660-4 |
| format | Article |
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β-amyloid (
βA) biologically active fragment
β-(25–35) involve oxidative stress. We examined the influence of culture conditions on the neuroprotective activity of antioxidants against
β-(25–35) toxicity. Common radical scavengers such as
N-acetylcysteine (250
μM) and
N-
t-butyl-phenylnitrone (500
μM) only protected cortical cells cultured in the presence of fetal calf serum (FCS) from
βA insult. The neuroprotective effect of lazaroids (U74389G and U83836E), 21-aminosteroids with antioxidant activity, was tested in cells grown with or without FCS. U74389G did not interfere with
β-(25–35) toxicity in either condition, while U83836E at a very low concentration (15 nM) protected cortical cells exposed to the
β peptide only when the neurons were cultured in the presence of FCS. These data show that a lazaroid can prevent
β-(25–35) toxicity and that the antioxidants exerted their protective effect in certain conditions.</description><identifier>ISSN: 0006-8993</identifier><identifier>EISSN: 1872-6240</identifier><identifier>DOI: 10.1016/S0006-8993(97)00660-4</identifier><identifier>PMID: 9295228</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Amyloid beta-Peptides - antagonists & inhibitors ; Amyloid beta-Peptides - toxicity ; Animals ; Antioxidants - pharmacology ; Cell Survival - drug effects ; Cells, Cultured ; Cerebral Cortex - cytology ; Cerebral Cortex - drug effects ; Chromans - pharmacology ; Cortical cell ; Free Radical Scavengers - pharmacology ; Lazaroid ; Neuroglia - drug effects ; Neuroglia - metabolism ; Neuroprotective Agents - pharmacology ; Oxidative stress ; Oxidative Stress - physiology ; Peptide Fragments - pharmacology ; Piperazines - pharmacology ; Pregnatrienes - pharmacology ; Rats ; β-Amyloid</subject><ispartof>Brain research, 1997-08, Vol.764 (1), p.293-298</ispartof><rights>1997 Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c391t-d0a150e164a9dcfaf5365231661755b7322b52b1f6506ff6651a653284b492c73</citedby><cites>FETCH-LOGICAL-c391t-d0a150e164a9dcfaf5365231661755b7322b52b1f6506ff6651a653284b492c73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0006-8993(97)00660-4$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9295228$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lucca, Elisa</creatorcontrib><creatorcontrib>Angeretti, Nadia</creatorcontrib><creatorcontrib>Forloni, Gianluigi</creatorcontrib><title>Influence of cell culture conditions on the protective effect of antioxidants against β-amyloid toxicity: studies with lazaroids</title><title>Brain research</title><addtitle>Brain Res</addtitle><description>The mechanisms of cell death of rat cortical neurons chronically exposed to the
β-amyloid (
βA) biologically active fragment
β-(25–35) involve oxidative stress. We examined the influence of culture conditions on the neuroprotective activity of antioxidants against
β-(25–35) toxicity. Common radical scavengers such as
N-acetylcysteine (250
μM) and
N-
t-butyl-phenylnitrone (500
μM) only protected cortical cells cultured in the presence of fetal calf serum (FCS) from
βA insult. The neuroprotective effect of lazaroids (U74389G and U83836E), 21-aminosteroids with antioxidant activity, was tested in cells grown with or without FCS. U74389G did not interfere with
β-(25–35) toxicity in either condition, while U83836E at a very low concentration (15 nM) protected cortical cells exposed to the
β peptide only when the neurons were cultured in the presence of FCS. These data show that a lazaroid can prevent
β-(25–35) toxicity and that the antioxidants exerted their protective effect in certain conditions.</description><subject>Amyloid beta-Peptides - antagonists & inhibitors</subject><subject>Amyloid beta-Peptides - toxicity</subject><subject>Animals</subject><subject>Antioxidants - pharmacology</subject><subject>Cell Survival - drug effects</subject><subject>Cells, Cultured</subject><subject>Cerebral Cortex - cytology</subject><subject>Cerebral Cortex - drug effects</subject><subject>Chromans - pharmacology</subject><subject>Cortical cell</subject><subject>Free Radical Scavengers - pharmacology</subject><subject>Lazaroid</subject><subject>Neuroglia - drug effects</subject><subject>Neuroglia - metabolism</subject><subject>Neuroprotective Agents - pharmacology</subject><subject>Oxidative stress</subject><subject>Oxidative Stress - physiology</subject><subject>Peptide Fragments - pharmacology</subject><subject>Piperazines - pharmacology</subject><subject>Pregnatrienes - pharmacology</subject><subject>Rats</subject><subject>β-Amyloid</subject><issn>0006-8993</issn><issn>1872-6240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1OHDEQhS2UCCbAEZC8QsmiiX_a7jGbKEIJICFlEVhbbrsMRj1tsN0kwy5XykFyJtzMiG1WVaX3qkrvQ-iIkhNKqPz8kxAim6VS_KPqPtVekqbdQQu67FgjWUveocWbZQ99yPm-jpwrsot2FVOCseUC_bkc_TDBaAFHjy0MA7bTUKYE2MbRhRLimHEccbkD_JBiAVvCE2Dwvnbzjhmr53dwtWZsbk0Yc8H__jZmtR5icLhU0YayPsW5TC5Axr9CucODeTap6vkAvfdmyHC4rfvo5vu367OL5urH-eXZ16vGckVL44ihggCVrVHOeuMFl4JxKiXthOg7zlgvWE-9FER6L6WgRgrOlm3fKmY7vo-ON3driscJctGrkOfAZoQ4ZU0lYfUer0axMdoUc07g9UMKK5PWmhI9o9ev6PXMVatOv6LXbd072j6Y-hW4t60t66p_2ehQUz4FSDrbMJN3IVWW2sXwnw8vtneV4A</recordid><startdate>19970801</startdate><enddate>19970801</enddate><creator>Lucca, Elisa</creator><creator>Angeretti, Nadia</creator><creator>Forloni, Gianluigi</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope></search><sort><creationdate>19970801</creationdate><title>Influence of cell culture conditions on the protective effect of antioxidants against β-amyloid toxicity: studies with lazaroids</title><author>Lucca, Elisa ; Angeretti, Nadia ; Forloni, Gianluigi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-d0a150e164a9dcfaf5365231661755b7322b52b1f6506ff6651a653284b492c73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Amyloid beta-Peptides - antagonists & inhibitors</topic><topic>Amyloid beta-Peptides - toxicity</topic><topic>Animals</topic><topic>Antioxidants - pharmacology</topic><topic>Cell Survival - drug effects</topic><topic>Cells, Cultured</topic><topic>Cerebral Cortex - cytology</topic><topic>Cerebral Cortex - drug effects</topic><topic>Chromans - pharmacology</topic><topic>Cortical cell</topic><topic>Free Radical Scavengers - pharmacology</topic><topic>Lazaroid</topic><topic>Neuroglia - drug effects</topic><topic>Neuroglia - metabolism</topic><topic>Neuroprotective Agents - pharmacology</topic><topic>Oxidative stress</topic><topic>Oxidative Stress - physiology</topic><topic>Peptide Fragments - pharmacology</topic><topic>Piperazines - pharmacology</topic><topic>Pregnatrienes - pharmacology</topic><topic>Rats</topic><topic>β-Amyloid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lucca, Elisa</creatorcontrib><creatorcontrib>Angeretti, Nadia</creatorcontrib><creatorcontrib>Forloni, Gianluigi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><jtitle>Brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lucca, Elisa</au><au>Angeretti, Nadia</au><au>Forloni, Gianluigi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Influence of cell culture conditions on the protective effect of antioxidants against β-amyloid toxicity: studies with lazaroids</atitle><jtitle>Brain research</jtitle><addtitle>Brain Res</addtitle><date>1997-08-01</date><risdate>1997</risdate><volume>764</volume><issue>1</issue><spage>293</spage><epage>298</epage><pages>293-298</pages><issn>0006-8993</issn><eissn>1872-6240</eissn><abstract>The mechanisms of cell death of rat cortical neurons chronically exposed to the
β-amyloid (
βA) biologically active fragment
β-(25–35) involve oxidative stress. We examined the influence of culture conditions on the neuroprotective activity of antioxidants against
β-(25–35) toxicity. Common radical scavengers such as
N-acetylcysteine (250
μM) and
N-
t-butyl-phenylnitrone (500
μM) only protected cortical cells cultured in the presence of fetal calf serum (FCS) from
βA insult. The neuroprotective effect of lazaroids (U74389G and U83836E), 21-aminosteroids with antioxidant activity, was tested in cells grown with or without FCS. U74389G did not interfere with
β-(25–35) toxicity in either condition, while U83836E at a very low concentration (15 nM) protected cortical cells exposed to the
β peptide only when the neurons were cultured in the presence of FCS. These data show that a lazaroid can prevent
β-(25–35) toxicity and that the antioxidants exerted their protective effect in certain conditions.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>9295228</pmid><doi>10.1016/S0006-8993(97)00660-4</doi><tpages>6</tpages></addata></record> |
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| ispartof | Brain research, 1997-08, Vol.764 (1), p.293-298 |
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| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Amyloid beta-Peptides - antagonists & inhibitors Amyloid beta-Peptides - toxicity Animals Antioxidants - pharmacology Cell Survival - drug effects Cells, Cultured Cerebral Cortex - cytology Cerebral Cortex - drug effects Chromans - pharmacology Cortical cell Free Radical Scavengers - pharmacology Lazaroid Neuroglia - drug effects Neuroglia - metabolism Neuroprotective Agents - pharmacology Oxidative stress Oxidative Stress - physiology Peptide Fragments - pharmacology Piperazines - pharmacology Pregnatrienes - pharmacology Rats β-Amyloid |
| title | Influence of cell culture conditions on the protective effect of antioxidants against β-amyloid toxicity: studies with lazaroids |
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