Influence of cell culture conditions on the protective effect of antioxidants against β-amyloid toxicity: studies with lazaroids

The mechanisms of cell death of rat cortical neurons chronically exposed to the β-amyloid ( βA) biologically active fragment β-(25–35) involve oxidative stress. We examined the influence of culture conditions on the neuroprotective activity of antioxidants against β-(25–35) toxicity. Common radical...

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Veröffentlicht in:Brain research 1997-08, Vol.764 (1), p.293-298
Hauptverfasser: Lucca, Elisa, Angeretti, Nadia, Forloni, Gianluigi
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Sprache:eng
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Zusammenfassung:The mechanisms of cell death of rat cortical neurons chronically exposed to the β-amyloid ( βA) biologically active fragment β-(25–35) involve oxidative stress. We examined the influence of culture conditions on the neuroprotective activity of antioxidants against β-(25–35) toxicity. Common radical scavengers such as N-acetylcysteine (250 μM) and N- t-butyl-phenylnitrone (500 μM) only protected cortical cells cultured in the presence of fetal calf serum (FCS) from βA insult. The neuroprotective effect of lazaroids (U74389G and U83836E), 21-aminosteroids with antioxidant activity, was tested in cells grown with or without FCS. U74389G did not interfere with β-(25–35) toxicity in either condition, while U83836E at a very low concentration (15 nM) protected cortical cells exposed to the β peptide only when the neurons were cultured in the presence of FCS. These data show that a lazaroid can prevent β-(25–35) toxicity and that the antioxidants exerted their protective effect in certain conditions.
ISSN:0006-8993
1872-6240
DOI:10.1016/S0006-8993(97)00660-4