Retron for the 67-Base Multicopy Single-Stranded DNA from Escherichia coli: A Potential Transposable Element Encoding Both Reverse Transcriptase and Dam Methylase Functions
The region (retron-Ec67) required for the biosynthesis of a branched-RNA-linked multicopy single-stranded DNA (msDNA-Ec67) from a clinical isolate of Escherichia coli was mapped at a position equivalent to 19 min on the K-12 chromosome. The element containing the retron consisted of a unique 34-kilo...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1990-12, Vol.87 (23), p.9454-9458 |
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| creator | Hsu, Mei-Yin Inouye, Masayori Inouye, Sumiko |
| description | The region (retron-Ec67) required for the biosynthesis of a branched-RNA-linked multicopy single-stranded DNA (msDNA-Ec67) from a clinical isolate of Escherichia coli was mapped at a position equivalent to 19 min on the K-12 chromosome. The element containing the retron consisted of a unique 34-kilobase sequence that was flanked by direct repeats of a 26-base-pair sequence found in the K-12 chromosomal DNA. This suggests that the 34-kilobase element was probably integrated into the E. coli genome by a mechanism related to transposition or phage integration. In the 34-kilobase sequence an open reading frame of 285 residues was found, which displays 44% sequence identity with the E. coli Dam methylase. Interestingly, there are three GATC sequences, the site of Dam methylation, in the promoter region of the gene for reverse transcriptase. |
| doi_str_mv | 10.1073/pnas.87.23.9454 |
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The element containing the retron consisted of a unique 34-kilobase sequence that was flanked by direct repeats of a 26-base-pair sequence found in the K-12 chromosomal DNA. This suggests that the 34-kilobase element was probably integrated into the E. coli genome by a mechanism related to transposition or phage integration. In the 34-kilobase sequence an open reading frame of 285 residues was found, which displays 44% sequence identity with the E. coli Dam methylase. Interestingly, there are three GATC sequences, the site of Dam methylation, in the promoter region of the gene for reverse transcriptase.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.87.23.9454</identifier><identifier>PMID: 1701261</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Amino Acid Sequence ; Amino acids ; Bacteriophages ; Base Sequence ; Biological and medical sciences ; Chromosome Mapping ; Chromosomes ; Chromosomes, Bacterial ; Dams ; DNA ; DNA Transposable Elements ; DNA, Single-Stranded - genetics ; Escherichia coli - genetics ; Escherichia coli Proteins ; Fundamental and applied biological sciences. Psychology ; Genes ; Genic rearrangement. Recombination. Transposable element ; Genomes ; Methyltransferases - genetics ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Nucleotide sequences ; Open reading frames ; Restriction Mapping ; RNA-Directed DNA Polymerase - genetics ; Sequence Homology, Nucleic Acid ; Site-Specific DNA-Methyltransferase (Adenine-Specific) ; Transposons</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1990-12, Vol.87 (23), p.9454-9458</ispartof><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c618t-f8cb1e98946da3c9d29abba12902903e3221839ed8bfc7f24871371219e0bc9b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/87/23.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2355841$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2355841$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27901,27902,53766,53768,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19627510$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1701261$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hsu, Mei-Yin</creatorcontrib><creatorcontrib>Inouye, Masayori</creatorcontrib><creatorcontrib>Inouye, Sumiko</creatorcontrib><title>Retron for the 67-Base Multicopy Single-Stranded DNA from Escherichia coli: A Potential Transposable Element Encoding Both Reverse Transcriptase and Dam Methylase Functions</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>The region (retron-Ec67) required for the biosynthesis of a branched-RNA-linked multicopy single-stranded DNA (msDNA-Ec67) from a clinical isolate of Escherichia coli was mapped at a position equivalent to 19 min on the K-12 chromosome. The element containing the retron consisted of a unique 34-kilobase sequence that was flanked by direct repeats of a 26-base-pair sequence found in the K-12 chromosomal DNA. This suggests that the 34-kilobase element was probably integrated into the E. coli genome by a mechanism related to transposition or phage integration. In the 34-kilobase sequence an open reading frame of 285 residues was found, which displays 44% sequence identity with the E. coli Dam methylase. Interestingly, there are three GATC sequences, the site of Dam methylation, in the promoter region of the gene for reverse transcriptase.</description><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>Bacteriophages</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Chromosome Mapping</subject><subject>Chromosomes</subject><subject>Chromosomes, Bacterial</subject><subject>Dams</subject><subject>DNA</subject><subject>DNA Transposable Elements</subject><subject>DNA, Single-Stranded - genetics</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli Proteins</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes</subject><subject>Genic rearrangement. Recombination. Transposable element</subject><subject>Genomes</subject><subject>Methyltransferases - genetics</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Nucleotide sequences</subject><subject>Open reading frames</subject><subject>Restriction Mapping</subject><subject>RNA-Directed DNA Polymerase - genetics</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Site-Specific DNA-Methyltransferase (Adenine-Specific)</subject><subject>Transposons</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkk9v1DAQxSMEKkvhzAWQL8ApW9v5ZyMu23YLSC2gtpwtx5k0rpw42E7Ffic-JA67tPQCkiVLfr-Z96yZJHlO8JLgKjsYB-mXrFrSbMnzIn-QLAjmJC1zjh8mC4xplbKc5o-TJ95fY4x5wfBeskcqTGhJFsnPcwjODqi1DoUOUFmlh9IDOptM0MqOG3ShhysD6UVwcmigQcefV6h1tkdrrzpwWnVaImWNfodW6KsNMAQtDbqMuB-tl7UBtDbQx3e0HpRtYj90aEOHzuEGXPT6jSqnxzA7Rxd0LHt0BqHbmPnlZBpU0HbwT5NHrTQenu3u_eTbyfry6GN6-uXDp6PVaapKwkLaMlUT4IznZSMzxRvKZV1LQjmOJ4OMUsIyDg2rW1W1NGcVySpCCQdcK15n-8n7bd9xqntoVIzupBGj0710G2GlFveVQXfiyt6IoiAsj-VvduXOfp_AB9Frr8AYOYCdvGCYFBWOSf4HkhJTHKNG8GALKme9d9DeZiFYzHsg5j0QrBI0E_MexIqXf3_hjt8OPuqvd7r0Spo2jkBpf4fxklYFmSO-2nGzwR_5ntHbfwKinYwJ8CNE8sWWvPbBuluUZkXBcpL9Am7h4BA</recordid><startdate>19901201</startdate><enddate>19901201</enddate><creator>Hsu, Mei-Yin</creator><creator>Inouye, Masayori</creator><creator>Inouye, Sumiko</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19901201</creationdate><title>Retron for the 67-Base Multicopy Single-Stranded DNA from Escherichia coli: A Potential Transposable Element Encoding Both Reverse Transcriptase and Dam Methylase Functions</title><author>Hsu, Mei-Yin ; Inouye, Masayori ; Inouye, Sumiko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c618t-f8cb1e98946da3c9d29abba12902903e3221839ed8bfc7f24871371219e0bc9b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Amino Acid Sequence</topic><topic>Amino acids</topic><topic>Bacteriophages</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Chromosome Mapping</topic><topic>Chromosomes</topic><topic>Chromosomes, Bacterial</topic><topic>Dams</topic><topic>DNA</topic><topic>DNA Transposable Elements</topic><topic>DNA, Single-Stranded - genetics</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli Proteins</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes</topic><topic>Genic rearrangement. Recombination. Transposable element</topic><topic>Genomes</topic><topic>Methyltransferases - genetics</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Nucleotide sequences</topic><topic>Open reading frames</topic><topic>Restriction Mapping</topic><topic>RNA-Directed DNA Polymerase - genetics</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>Site-Specific DNA-Methyltransferase (Adenine-Specific)</topic><topic>Transposons</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hsu, Mei-Yin</creatorcontrib><creatorcontrib>Inouye, Masayori</creatorcontrib><creatorcontrib>Inouye, Sumiko</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hsu, Mei-Yin</au><au>Inouye, Masayori</au><au>Inouye, Sumiko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Retron for the 67-Base Multicopy Single-Stranded DNA from Escherichia coli: A Potential Transposable Element Encoding Both Reverse Transcriptase and Dam Methylase Functions</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1990-12-01</date><risdate>1990</risdate><volume>87</volume><issue>23</issue><spage>9454</spage><epage>9458</epage><pages>9454-9458</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>The region (retron-Ec67) required for the biosynthesis of a branched-RNA-linked multicopy single-stranded DNA (msDNA-Ec67) from a clinical isolate of Escherichia coli was mapped at a position equivalent to 19 min on the K-12 chromosome. The element containing the retron consisted of a unique 34-kilobase sequence that was flanked by direct repeats of a 26-base-pair sequence found in the K-12 chromosomal DNA. This suggests that the 34-kilobase element was probably integrated into the E. coli genome by a mechanism related to transposition or phage integration. In the 34-kilobase sequence an open reading frame of 285 residues was found, which displays 44% sequence identity with the E. coli Dam methylase. Interestingly, there are three GATC sequences, the site of Dam methylation, in the promoter region of the gene for reverse transcriptase.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>1701261</pmid><doi>10.1073/pnas.87.23.9454</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Amino Acid Sequence Amino acids Bacteriophages Base Sequence Biological and medical sciences Chromosome Mapping Chromosomes Chromosomes, Bacterial Dams DNA DNA Transposable Elements DNA, Single-Stranded - genetics Escherichia coli - genetics Escherichia coli Proteins Fundamental and applied biological sciences. Psychology Genes Genic rearrangement. Recombination. Transposable element Genomes Methyltransferases - genetics Molecular and cellular biology Molecular genetics Molecular Sequence Data Nucleotide sequences Open reading frames Restriction Mapping RNA-Directed DNA Polymerase - genetics Sequence Homology, Nucleic Acid Site-Specific DNA-Methyltransferase (Adenine-Specific) Transposons |
| title | Retron for the 67-Base Multicopy Single-Stranded DNA from Escherichia coli: A Potential Transposable Element Encoding Both Reverse Transcriptase and Dam Methylase Functions |
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