Effects of various carbon and nitrogen sources on the biosynthesis of extracellular acidic proteinases of Penicillium roqueforti

The effect of various nitrogen and carbon sources on the biosynthesis of extracellular acid proteases by Penicillium roqueforti was followed. The enzymes were partly purified by (NH4)2SO4 precipitation and dialysis. At 26 degrees C and initial pH 4.0, the largest enzymatic activity was obtained afte...

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Veröffentlicht in:Biotechnology letters 1991, Vol.13 (6), p.451-454
Hauptverfasser: Petrovic, S.E, Becarevic, A, Banka, L, Zagar, Z
Format: Artikel
Sprache:eng
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Zusammenfassung:The effect of various nitrogen and carbon sources on the biosynthesis of extracellular acid proteases by Penicillium roqueforti was followed. The enzymes were partly purified by (NH4)2SO4 precipitation and dialysis. At 26 degrees C and initial pH 4.0, the largest enzymatic activity was obtained after 48 hours of growth in the presence of 0.15% of urea and 2.0% of Na-citrate in nutrient broth.
ISSN:0141-5492
1573-6776
DOI:10.1007/BF01031000