Effects of various carbon and nitrogen sources on the biosynthesis of extracellular acidic proteinases of Penicillium roqueforti
The effect of various nitrogen and carbon sources on the biosynthesis of extracellular acid proteases by Penicillium roqueforti was followed. The enzymes were partly purified by (NH4)2SO4 precipitation and dialysis. At 26 degrees C and initial pH 4.0, the largest enzymatic activity was obtained afte...
Gespeichert in:
Veröffentlicht in: | Biotechnology letters 1991, Vol.13 (6), p.451-454 |
---|---|
Hauptverfasser: | , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | The effect of various nitrogen and carbon sources on the biosynthesis of extracellular acid proteases by Penicillium roqueforti was followed. The enzymes were partly purified by (NH4)2SO4 precipitation and dialysis. At 26 degrees C and initial pH 4.0, the largest enzymatic activity was obtained after 48 hours of growth in the presence of 0.15% of urea and 2.0% of Na-citrate in nutrient broth. |
---|---|
ISSN: | 0141-5492 1573-6776 |
DOI: | 10.1007/BF01031000 |