Expression cloning of a rat B sub(2) bradykinin receptor
A cDNA encoding a functional bradykinin receptor was isolated from a rat uterus library by a clonal selection strategy using Xenopus laevis oocytes to assay for expression of bradykinin responses. The predicted protein is homologous to the seven transmembrane G protein-coupled superfamily of recepto...
Gespeichert in:
| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1991-01, Vol.88 (17), p.7724-7728 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 7728 |
|---|---|
| container_issue | 17 |
| container_start_page | 7724 |
| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 88 |
| creator | McEachern, A E Shelton, E R Bhakta, S Obernolte, R Bach, C Zuppan, P Fujisaki, J Aldrich, R W Jarnagin, K |
| description | A cDNA encoding a functional bradykinin receptor was isolated from a rat uterus library by a clonal selection strategy using Xenopus laevis oocytes to assay for expression of bradykinin responses. The predicted protein is homologous to the seven transmembrane G protein-coupled superfamily of receptors. Bradykinin and its analogs stimulate a Cl super(-) current in oocytes expressing the receptor with the rank order of potency: bradykinin approximately equals Lys-bradykinin > (Tyr super(8))-bradykinin >> (Phe super(6))bradykinin. This is the rank order of potency observed for these compounds in competitive binding assays on soluble receptor from rat uterus. |
| format | Article |
| fullrecord | <record><control><sourceid>proquest</sourceid><recordid>TN_cdi_proquest_miscellaneous_15992956</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>15992956</sourcerecordid><originalsourceid>FETCH-proquest_miscellaneous_159929563</originalsourceid><addsrcrecordid>eNqNyr0OwiAUQGEGTaw_73Ano0MTikXLqqnxAdwbircGRajckujb6-ADOJ3hOyOWcS52eVWKcsKmRDfOuZIVz1hVv_qIRDZ4MC54668QOtAQ9QB7oNSuxBraqC_vu_0qRDTYDyHO2bjTjnDx64wtj_X5cMr7GJ4JaWgelgw6pz2GRE0hlRJKbjd_jx_2ujgJ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15992956</pqid></control><display><type>article</type><title>Expression cloning of a rat B sub(2) bradykinin receptor</title><source>JSTOR Archive Collection A-Z Listing</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><creator>McEachern, A E ; Shelton, E R ; Bhakta, S ; Obernolte, R ; Bach, C ; Zuppan, P ; Fujisaki, J ; Aldrich, R W ; Jarnagin, K</creator><creatorcontrib>McEachern, A E ; Shelton, E R ; Bhakta, S ; Obernolte, R ; Bach, C ; Zuppan, P ; Fujisaki, J ; Aldrich, R W ; Jarnagin, K</creatorcontrib><description>A cDNA encoding a functional bradykinin receptor was isolated from a rat uterus library by a clonal selection strategy using Xenopus laevis oocytes to assay for expression of bradykinin responses. The predicted protein is homologous to the seven transmembrane G protein-coupled superfamily of receptors. Bradykinin and its analogs stimulate a Cl super(-) current in oocytes expressing the receptor with the rank order of potency: bradykinin approximately equals Lys-bradykinin > (Tyr super(8))-bradykinin >> (Phe super(6))bradykinin. This is the rank order of potency observed for these compounds in competitive binding assays on soluble receptor from rat uterus.</description><identifier>ISSN: 0027-8424</identifier><language>eng</language><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1991-01, Vol.88 (17), p.7724-7728</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids></links><search><creatorcontrib>McEachern, A E</creatorcontrib><creatorcontrib>Shelton, E R</creatorcontrib><creatorcontrib>Bhakta, S</creatorcontrib><creatorcontrib>Obernolte, R</creatorcontrib><creatorcontrib>Bach, C</creatorcontrib><creatorcontrib>Zuppan, P</creatorcontrib><creatorcontrib>Fujisaki, J</creatorcontrib><creatorcontrib>Aldrich, R W</creatorcontrib><creatorcontrib>Jarnagin, K</creatorcontrib><title>Expression cloning of a rat B sub(2) bradykinin receptor</title><title>Proceedings of the National Academy of Sciences - PNAS</title><description>A cDNA encoding a functional bradykinin receptor was isolated from a rat uterus library by a clonal selection strategy using Xenopus laevis oocytes to assay for expression of bradykinin responses. The predicted protein is homologous to the seven transmembrane G protein-coupled superfamily of receptors. Bradykinin and its analogs stimulate a Cl super(-) current in oocytes expressing the receptor with the rank order of potency: bradykinin approximately equals Lys-bradykinin > (Tyr super(8))-bradykinin >> (Phe super(6))bradykinin. This is the rank order of potency observed for these compounds in competitive binding assays on soluble receptor from rat uterus.</description><issn>0027-8424</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><recordid>eNqNyr0OwiAUQGEGTaw_73Ano0MTikXLqqnxAdwbircGRajckujb6-ADOJ3hOyOWcS52eVWKcsKmRDfOuZIVz1hVv_qIRDZ4MC54668QOtAQ9QB7oNSuxBraqC_vu_0qRDTYDyHO2bjTjnDx64wtj_X5cMr7GJ4JaWgelgw6pz2GRE0hlRJKbjd_jx_2ujgJ</recordid><startdate>19910101</startdate><enddate>19910101</enddate><creator>McEachern, A E</creator><creator>Shelton, E R</creator><creator>Bhakta, S</creator><creator>Obernolte, R</creator><creator>Bach, C</creator><creator>Zuppan, P</creator><creator>Fujisaki, J</creator><creator>Aldrich, R W</creator><creator>Jarnagin, K</creator><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M81</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>19910101</creationdate><title>Expression cloning of a rat B sub(2) bradykinin receptor</title><author>McEachern, A E ; Shelton, E R ; Bhakta, S ; Obernolte, R ; Bach, C ; Zuppan, P ; Fujisaki, J ; Aldrich, R W ; Jarnagin, K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-proquest_miscellaneous_159929563</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>McEachern, A E</creatorcontrib><creatorcontrib>Shelton, E R</creatorcontrib><creatorcontrib>Bhakta, S</creatorcontrib><creatorcontrib>Obernolte, R</creatorcontrib><creatorcontrib>Bach, C</creatorcontrib><creatorcontrib>Zuppan, P</creatorcontrib><creatorcontrib>Fujisaki, J</creatorcontrib><creatorcontrib>Aldrich, R W</creatorcontrib><creatorcontrib>Jarnagin, K</creatorcontrib><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>McEachern, A E</au><au>Shelton, E R</au><au>Bhakta, S</au><au>Obernolte, R</au><au>Bach, C</au><au>Zuppan, P</au><au>Fujisaki, J</au><au>Aldrich, R W</au><au>Jarnagin, K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression cloning of a rat B sub(2) bradykinin receptor</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><date>1991-01-01</date><risdate>1991</risdate><volume>88</volume><issue>17</issue><spage>7724</spage><epage>7728</epage><pages>7724-7728</pages><issn>0027-8424</issn><abstract>A cDNA encoding a functional bradykinin receptor was isolated from a rat uterus library by a clonal selection strategy using Xenopus laevis oocytes to assay for expression of bradykinin responses. The predicted protein is homologous to the seven transmembrane G protein-coupled superfamily of receptors. Bradykinin and its analogs stimulate a Cl super(-) current in oocytes expressing the receptor with the rank order of potency: bradykinin approximately equals Lys-bradykinin > (Tyr super(8))-bradykinin >> (Phe super(6))bradykinin. This is the rank order of potency observed for these compounds in competitive binding assays on soluble receptor from rat uterus.</abstract></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1991-01, Vol.88 (17), p.7724-7728 |
| issn | 0027-8424 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_15992956 |
| source | JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| title | Expression cloning of a rat B sub(2) bradykinin receptor |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T20%3A30%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Expression%20cloning%20of%20a%20rat%20B%20sub(2)%20bradykinin%20receptor&rft.jtitle=Proceedings%20of%20the%20National%20Academy%20of%20Sciences%20-%20PNAS&rft.au=McEachern,%20A%20E&rft.date=1991-01-01&rft.volume=88&rft.issue=17&rft.spage=7724&rft.epage=7728&rft.pages=7724-7728&rft.issn=0027-8424&rft_id=info:doi/&rft_dat=%3Cproquest%3E15992956%3C/proquest%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=15992956&rft_id=info:pmid/&rfr_iscdi=true |