Expression cloning of a rat B sub(2) bradykinin receptor

A cDNA encoding a functional bradykinin receptor was isolated from a rat uterus library by a clonal selection strategy using Xenopus laevis oocytes to assay for expression of bradykinin responses. The predicted protein is homologous to the seven transmembrane G protein-coupled superfamily of receptors. Bradykinin and its analogs stimulate a Cl super(-) current in oocytes expressing the receptor with the rank order of potency: bradykinin approximately equals Lys-bradykinin > (Tyr super(8))-bradykinin >> (Phe super(6))bradykinin. This is the rank order of potency observed for these compounds in competitive binding assays on soluble receptor from rat uterus.