Properties of isocitrate lyase from Escherichia coli K12 grown on acetate or glycolate

Properties of isocitrate lyase from Escherichia coli , the first enzyme of the glyoxylate bypass, have been compared from cells grown on either acetate or glycolate as the sole carbon source. Michaelis constants for isocitrate, isoelectric points, native and subunit molecular weights, antigenic properties, peptide mapping with V-8 or trypsin, and several other properties were examined. Our data suggest that only one isocitrate lyase from exists in E. coli regardless of carbon source used for growth.