A rapid tissue culture assay for the detection of okadaic acid and related compounds in mussels

Okadaic acid, the main toxin of the diarrhoetic shellfish poisoning (DSP) group, shows high cytotoxicity to Buffalo green monkey (BGM) kidney cell cultures after different times according to concentration. A rapid method of detecting the presence of okadaic acid and related compounds in contaminated...

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Veröffentlicht in:	Toxicon (Oxford) 1997-02, Vol.35 (2), p.223-230
Hauptverfasser:	Croci, Luciana, Cozzi, Loredana, Stacchini, Alessandro, De Medici, Dario, Toti, Laura
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals bioassays Biological and medical sciences Bivalvia cell lines Cercopithecus aethiops Culture Techniques cytotoxicity detection dinophyta food contamination Injections, Intraperitoneal Kidney - drug effects Kidney - pathology kidneys Marine Marine Toxins - analysis Marine Toxins - toxicity Medical sciences Mice monkeys mussels Mytilus galloprovincialis Okadaic Acid - analysis Okadaic Acid - toxicity Plant poisons toxicology testing tissue culture Toxicology toxins
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Beschreibung
Zusammenfassung:	Okadaic acid, the main toxin of the diarrhoetic shellfish poisoning (DSP) group, shows high cytotoxicity to Buffalo green monkey (BGM) kidney cell cultures after different times according to concentration. A rapid method of detecting the presence of okadaic acid and related compounds in contaminated mussels by means of a cytotoxicity test is presented. The method is based on direct microscopic observation of toxin-induced morphological changes in BGM cell cultures. A high correlation ( r = 0.950; P < 0.001) was found between the official mouse bioassay and the cytotoxicity test conducted on naturally contaminated samples of Mytilus galloprovincialis.
ISSN:	0041-0101 1879-3150
DOI:	10.1016/S0041-0101(96)00124-9