Some approaches to adaptive control of fed-batch culture of the cellobiase producer aspergillus japonicus

Biosynthesis of cellobiase by an Aspergillus japonicus culture under various modes of cultivation was studied using a fermenter‐computer system. Two modes of fed‐batch cultivation were developed: temperate and intensive. Both modes used the double algorithm of glucose supply based on the process control by the preset profile of CO2 concentration in exhaust gas and consumption of the pH stabilizing titrant. Intensive fed‐batch proved the most efficient mode: it maintained conditions for derepressed cellobiase Liosynthesis and decreased the carbon and energy limit for enzyme production. Intensive fed‐batch is, thus, the most adapted to cellobiase biosynthesis conditions and nutrient requirements of the producing culture.