Purification of blackgram mottle virus (BMoV) using magnesium-bentonite

Blackgram mottle virus was purified from French bean ( Phaseolus vulgaris) cv. Contender tissue using two different methods involving differential centrifugation. The use of Mg-bentonite during purification produced a homogeneous and pure virus preparation. The M r of RNA and coat protein were 1400...

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Veröffentlicht in:	Phytochemistry (Oxford) 1997-04, Vol.44 (7), p.1237-1240
Hauptverfasser:	Balasubrahmanyam, A., Kapoor, H.C., Varma, A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences blackgram mottle virus Fundamental and applied biological sciences. Psychology Phytopathology. Animal pests. Plant and forest protection Plant viruses and viroids Systematics. Structure, properties and multiplication. Genetics
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Beschreibung
Zusammenfassung:	Blackgram mottle virus was purified from French bean ( Phaseolus vulgaris) cv. Contender tissue using two different methods involving differential centrifugation. The use of Mg-bentonite during purification produced a homogeneous and pure virus preparation. The M r of RNA and coat protein were 1400 and 39k, respectively. The RNA was infectious at a concentration as low as 7 μg ml −1.
ISSN:	0031-9422 1873-3700
DOI:	10.1016/S0031-9422(96)00740-6