Methods for in vitro skin absorption studies of a lipophilic toxin produced by red tide
The penetration and distribution of [ 3H]PbTx-3 into pig skin was determined using in vivo and in vitro methods. The dose used in each topical study was 0.3–0.4 μg/cm 2 skin, with dimethylsulfoxide as the vehicle. In the in vivo study, mean cutaneous absorption after 48 h (expressed as percentage of...
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| Veröffentlicht in: | Toxicology (Amsterdam) 1991-02, Vol.66 (1), p.1-17 |
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| creator | Kemppainen, Barbara W. Reifenrath, William G. Stafford, Robert G. Mehta, Meena |
| description | The penetration and distribution of [
3H]PbTx-3 into pig skin was determined using in vivo and in vitro methods. The dose used in each topical study was 0.3–0.4 μg/cm
2 skin, with dimethylsulfoxide as the vehicle. In the in vivo study, mean cutaneous absorption after 48 h (expressed as percentage of the dose) was 11.5% (
n = 3). In the in vitro study, mean cutaneous absorption after 48 h was 1.6% (
n = 12), when based on accumulation of radioactivity in receptor fluid, or 9.9% when based on receptor fluid and dermis. [
3H]PbTx-3 readily penetrated through the epidermis into the dermis, reaching maximal dermal accumulation at 4 h (9.1% in vivo and 18% in vitro). At 24 h, the amount in the dermis decreased to 2.3% and 15% in vivo and in vitro, respectively and at 48 h the amount in the dermis decreased to 8.2% in vitro. These results demonstrate the important role of the dermis as a reservoir for a lipophilic compound in both in vivo and in vitro percutaneous absorption studies. |
| doi_str_mv | 10.1016/0300-483X(91)90174-Y |
| format | Article |
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3H]PbTx-3 into pig skin was determined using in vivo and in vitro methods. The dose used in each topical study was 0.3–0.4 μg/cm
2 skin, with dimethylsulfoxide as the vehicle. In the in vivo study, mean cutaneous absorption after 48 h (expressed as percentage of the dose) was 11.5% (
n = 3). In the in vitro study, mean cutaneous absorption after 48 h was 1.6% (
n = 12), when based on accumulation of radioactivity in receptor fluid, or 9.9% when based on receptor fluid and dermis. [
3H]PbTx-3 readily penetrated through the epidermis into the dermis, reaching maximal dermal accumulation at 4 h (9.1% in vivo and 18% in vitro). At 24 h, the amount in the dermis decreased to 2.3% and 15% in vivo and in vitro, respectively and at 48 h the amount in the dermis decreased to 8.2% in vitro. These results demonstrate the important role of the dermis as a reservoir for a lipophilic compound in both in vivo and in vitro percutaneous absorption studies.</description><identifier>ISSN: 0300-483X</identifier><identifier>EISSN: 1879-3185</identifier><identifier>DOI: 10.1016/0300-483X(91)90174-Y</identifier><identifier>PMID: 1996463</identifier><identifier>CODEN: TXICDD</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Administration, Topical ; Animals ; Biological and medical sciences ; Brevetoxin ; Female ; In vitro ; In vivo ; Male ; Marine ; Marine Toxins - pharmacokinetics ; Medical sciences ; Oxocins ; Plant poisons toxicology ; Ptychodiscus brevis ; Skin Absorption ; Skin penetration ; Species Specificity ; Swine ; Tissue Distribution ; Toxicology ; Tritium</subject><ispartof>Toxicology (Amsterdam), 1991-02, Vol.66 (1), p.1-17</ispartof><rights>1991</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c333t-c6092155e1e516e37aa1392628b127e413a94bd950d9149f959678f14b3bc41d3</citedby><cites>FETCH-LOGICAL-c333t-c6092155e1e516e37aa1392628b127e413a94bd950d9149f959678f14b3bc41d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0300-483X(91)90174-Y$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19497249$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1996463$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kemppainen, Barbara W.</creatorcontrib><creatorcontrib>Reifenrath, William G.</creatorcontrib><creatorcontrib>Stafford, Robert G.</creatorcontrib><creatorcontrib>Mehta, Meena</creatorcontrib><title>Methods for in vitro skin absorption studies of a lipophilic toxin produced by red tide</title><title>Toxicology (Amsterdam)</title><addtitle>Toxicology</addtitle><description>The penetration and distribution of [
3H]PbTx-3 into pig skin was determined using in vivo and in vitro methods. The dose used in each topical study was 0.3–0.4 μg/cm
2 skin, with dimethylsulfoxide as the vehicle. In the in vivo study, mean cutaneous absorption after 48 h (expressed as percentage of the dose) was 11.5% (
n = 3). In the in vitro study, mean cutaneous absorption after 48 h was 1.6% (
n = 12), when based on accumulation of radioactivity in receptor fluid, or 9.9% when based on receptor fluid and dermis. [
3H]PbTx-3 readily penetrated through the epidermis into the dermis, reaching maximal dermal accumulation at 4 h (9.1% in vivo and 18% in vitro). At 24 h, the amount in the dermis decreased to 2.3% and 15% in vivo and in vitro, respectively and at 48 h the amount in the dermis decreased to 8.2% in vitro. These results demonstrate the important role of the dermis as a reservoir for a lipophilic compound in both in vivo and in vitro percutaneous absorption studies.</description><subject>Administration, Topical</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Brevetoxin</subject><subject>Female</subject><subject>In vitro</subject><subject>In vivo</subject><subject>Male</subject><subject>Marine</subject><subject>Marine Toxins - pharmacokinetics</subject><subject>Medical sciences</subject><subject>Oxocins</subject><subject>Plant poisons toxicology</subject><subject>Ptychodiscus brevis</subject><subject>Skin Absorption</subject><subject>Skin penetration</subject><subject>Species Specificity</subject><subject>Swine</subject><subject>Tissue Distribution</subject><subject>Toxicology</subject><subject>Tritium</subject><issn>0300-483X</issn><issn>1879-3185</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMFu1DAQhi1EVbaFN2glX0BwCHhix4kvSKiigNSql1ZtT5ZjT1RDNg62U7Vvj7e7Ak49zUjzza9fHyFHwD4CA_mJccYq0fGb9wo-KAatqG5fkBV0rao4dM1LsvqLvCIHKf1kjNVcyH2yD0pJIfmKXJ9jvgsu0SFE6id673MMNP0qq-lTiHP2YaIpL85jomGgho5-DvOdH72lOTwUcI7BLRYd7R9pLCN7h6_J3mDGhG9285BcnX69PPlenV18-3Hy5ayynPNcWclUDU2DgA1I5K0xwFUt666HukUB3CjRO9Uwp0CoQTVKtt0Aoue9FeD4IXm3zS0lfi-Ysl77ZHEczYRhSRoaxWUt6gKKLWhjSCnioOfo1yY-amB641NvZOmNLK1AP_nUt-XteJe_9Gt0_562Asv97e5ukjXjEM1kffoPE6qthSrc5y2HRca9x6iT9TgVaz6izdoF_3yRP_0HkTk</recordid><startdate>19910211</startdate><enddate>19910211</enddate><creator>Kemppainen, Barbara W.</creator><creator>Reifenrath, William G.</creator><creator>Stafford, Robert G.</creator><creator>Mehta, Meena</creator><general>Elsevier Ireland Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope><scope>F1W</scope><scope>H97</scope><scope>L.G</scope><scope>M7N</scope></search><sort><creationdate>19910211</creationdate><title>Methods for in vitro skin absorption studies of a lipophilic toxin produced by red tide</title><author>Kemppainen, Barbara W. ; Reifenrath, William G. ; Stafford, Robert G. ; Mehta, Meena</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c333t-c6092155e1e516e37aa1392628b127e413a94bd950d9149f959678f14b3bc41d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Administration, Topical</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Brevetoxin</topic><topic>Female</topic><topic>In vitro</topic><topic>In vivo</topic><topic>Male</topic><topic>Marine</topic><topic>Marine Toxins - pharmacokinetics</topic><topic>Medical sciences</topic><topic>Oxocins</topic><topic>Plant poisons toxicology</topic><topic>Ptychodiscus brevis</topic><topic>Skin Absorption</topic><topic>Skin penetration</topic><topic>Species Specificity</topic><topic>Swine</topic><topic>Tissue Distribution</topic><topic>Toxicology</topic><topic>Tritium</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kemppainen, Barbara W.</creatorcontrib><creatorcontrib>Reifenrath, William G.</creatorcontrib><creatorcontrib>Stafford, Robert G.</creatorcontrib><creatorcontrib>Mehta, Meena</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Toxicology (Amsterdam)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kemppainen, Barbara W.</au><au>Reifenrath, William G.</au><au>Stafford, Robert G.</au><au>Mehta, Meena</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Methods for in vitro skin absorption studies of a lipophilic toxin produced by red tide</atitle><jtitle>Toxicology (Amsterdam)</jtitle><addtitle>Toxicology</addtitle><date>1991-02-11</date><risdate>1991</risdate><volume>66</volume><issue>1</issue><spage>1</spage><epage>17</epage><pages>1-17</pages><issn>0300-483X</issn><eissn>1879-3185</eissn><coden>TXICDD</coden><abstract>The penetration and distribution of [
3H]PbTx-3 into pig skin was determined using in vivo and in vitro methods. The dose used in each topical study was 0.3–0.4 μg/cm
2 skin, with dimethylsulfoxide as the vehicle. In the in vivo study, mean cutaneous absorption after 48 h (expressed as percentage of the dose) was 11.5% (
n = 3). In the in vitro study, mean cutaneous absorption after 48 h was 1.6% (
n = 12), when based on accumulation of radioactivity in receptor fluid, or 9.9% when based on receptor fluid and dermis. [
3H]PbTx-3 readily penetrated through the epidermis into the dermis, reaching maximal dermal accumulation at 4 h (9.1% in vivo and 18% in vitro). At 24 h, the amount in the dermis decreased to 2.3% and 15% in vivo and in vitro, respectively and at 48 h the amount in the dermis decreased to 8.2% in vitro. These results demonstrate the important role of the dermis as a reservoir for a lipophilic compound in both in vivo and in vitro percutaneous absorption studies.</abstract><cop>Shannon</cop><cop>Amsterdam</cop><pub>Elsevier Ireland Ltd</pub><pmid>1996463</pmid><doi>10.1016/0300-483X(91)90174-Y</doi><tpages>17</tpages></addata></record> |
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| ispartof | Toxicology (Amsterdam), 1991-02, Vol.66 (1), p.1-17 |
| issn | 0300-483X 1879-3185 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Administration, Topical Animals Biological and medical sciences Brevetoxin Female In vitro In vivo Male Marine Marine Toxins - pharmacokinetics Medical sciences Oxocins Plant poisons toxicology Ptychodiscus brevis Skin Absorption Skin penetration Species Specificity Swine Tissue Distribution Toxicology Tritium |
| title | Methods for in vitro skin absorption studies of a lipophilic toxin produced by red tide |
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