Methods for in vitro skin absorption studies of a lipophilic toxin produced by red tide

The penetration and distribution of [ 3H]PbTx-3 into pig skin was determined using in vivo and in vitro methods. The dose used in each topical study was 0.3–0.4 μg/cm 2 skin, with dimethylsulfoxide as the vehicle. In the in vivo study, mean cutaneous absorption after 48 h (expressed as percentage of...

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Veröffentlicht in:Toxicology (Amsterdam) 1991-02, Vol.66 (1), p.1-17
Hauptverfasser: Kemppainen, Barbara W., Reifenrath, William G., Stafford, Robert G., Mehta, Meena
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Sprache:eng
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Zusammenfassung:The penetration and distribution of [ 3H]PbTx-3 into pig skin was determined using in vivo and in vitro methods. The dose used in each topical study was 0.3–0.4 μg/cm 2 skin, with dimethylsulfoxide as the vehicle. In the in vivo study, mean cutaneous absorption after 48 h (expressed as percentage of the dose) was 11.5% ( n = 3). In the in vitro study, mean cutaneous absorption after 48 h was 1.6% ( n = 12), when based on accumulation of radioactivity in receptor fluid, or 9.9% when based on receptor fluid and dermis. [ 3H]PbTx-3 readily penetrated through the epidermis into the dermis, reaching maximal dermal accumulation at 4 h (9.1% in vivo and 18% in vitro). At 24 h, the amount in the dermis decreased to 2.3% and 15% in vivo and in vitro, respectively and at 48 h the amount in the dermis decreased to 8.2% in vitro. These results demonstrate the important role of the dermis as a reservoir for a lipophilic compound in both in vivo and in vitro percutaneous absorption studies.
ISSN:0300-483X
1879-3185
DOI:10.1016/0300-483X(91)90174-Y